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Cell-accurate optical mapping across the entire developing heart

Michael Weber, Nico Scherf, Peter Kohl, View ORCID ProfileJan Huisken
doi: https://doi.org/10.1101/143057
Michael Weber
1Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
2Harvard Medical School, Boston, MA 02115, USA
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  • For correspondence: jhuisken@morgridge.org scherf@cbs.mpg.de.
Nico Scherf
1Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
3Max Planck Institute for Human Cognitive and Brain Sciences, 04103 Leipzig, Germany
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  • For correspondence: jhuisken@morgridge.org scherf@cbs.mpg.de.
Peter Kohl
4Institute for Experimental Cardiovascular Medicine, University Heart Centre Freiburg Bad Krozingen, School of Medicine, Albert-Ludwigs University, 79110 Freiburg, Germany
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Jan Huisken
1Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
5Morgridge Institute for Research, Madison, WI 53715, USA
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  • ORCID record for Jan Huisken
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Abstract

Organogenesis depends on orchestrated interactions between individual cells and morphogenically relevant cues at the tissue level. This is true for the heart, whose function critically relies on well-ordered communication between neighbouring cells, which is established and fine-tuned during development. For an integrated understanding of the development of structure and function, we need to move from isolated snap-shot observations of either microscopic or macroscopic parameters to simultaneous and, ideally continuous, cell-to-organ scale imaging. We introduce cell-accurate three-dimensional Ca2+-mapping of all cells in the entire heart during the looping stage in live embryonic zebrafish, using high-speed light sheet microscopy and tailored image processing and analysis. We show how myocardial region-specific heterogeneity in cell function emerges during early development and how structural patterning goes hand-in-hand with functional maturation of the entire heart. Our method opens the way to systematic, scale-bridging, in vivo studies of vertebrate organogenesis by cell-accurate structure-function mapping across entire organs.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 27, 2017.
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Cell-accurate optical mapping across the entire developing heart
Michael Weber, Nico Scherf, Peter Kohl, Jan Huisken
bioRxiv 143057; doi: https://doi.org/10.1101/143057
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Cell-accurate optical mapping across the entire developing heart
Michael Weber, Nico Scherf, Peter Kohl, Jan Huisken
bioRxiv 143057; doi: https://doi.org/10.1101/143057

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