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Inoculation of biocontrol bacteria alleviated Panax ginseng replanting problem

Linlin Dong, Jiang Xu, Lianjuan Zhang, Guangfei Wei, He Su, Juan Yang, Jun Qian, Ran Xu, Baosheng Liao, Liang Shen, Mingli Wu, Ruiyang Cheng, Shilin Chen
doi: https://doi.org/10.1101/143412
Linlin Dong
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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Jiang Xu
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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Lianjuan Zhang
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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Guangfei Wei
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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He Su
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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Juan Yang
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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Jun Qian
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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Ran Xu
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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Baosheng Liao
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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Liang Shen
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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Mingli Wu
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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Ruiyang Cheng
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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Shilin Chen
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
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  • For correspondence: slchen@icmm.ac.cn
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Abstract

Replanting problem is a common and serious issue hindering the continuous cultivation of Panax plants. Changes in soil microbial community driven by plant species of different ages and developmental stages are speculated to cause this problem. Inoculation of microbial antagonists is proposed to alleviate replanting issues efficiently.

High-throughput sequencing revealed that bacterial diversity evidently decreased, and fungal diversity markedly increased in soils of adult ginseng plants in the root growth stage. Relatively few beneficial microbe agents, such as Luteolibacter, Cytophagaceae, Luteibacter, Sphingomonas, Sphingomonadaceae, and Zygomycota, were observed. On the contrary, the relative abundance of harmful microorganism agents, namely, Brevundimonas, Enterobacteriaceae, Pandoraea, Cantharellales, Dendryphion, Fusarium, and Chytridiomycota, increased with pant age. Furthermore, Bacillus subtilis 50-1 was isolated and served as microbial antagonists against pathogenic Fusarium oxysporum of ginseng root-rot, and its biocontrol efficacy was 67.8% using a dual culture assay. The ginseng death rate and relative abundance of Fusarium decreased by 63.3% and 46.1%, respectively, after inoculation with 50-1 in replanting soils. Data revealed that changes in the diversity and composition of rhizospheric microbial communities driven by ginseng of different ages and developmental stages could cause microecological degradation. Biocontrol using microbial antagonists was an effective method for alleviating the replanting problem.

Highlight Changes in rhizospheric microbial communities driven by ginseng plants 13 of different ages and developmental stages could cause microecological degradation. 14 Biocontrol using microbial antagonists effectively alleviated the replanting problem.

Footnotes

  • Linlin Dong E-mail: lldong{at}icmm.ac.cn; Jiang Xu E-mail: jxu{at}icmm.ac.cn; Lianjuan Zhang E-mail: zlj0970{at}163.com; Guangfei Wei E-mail: 15098855936{at}163.com; He Su E-mail: suhe.gd{at}qq.com; Juang Yang E-mail: yangjuan6263{at}163.com; Jun Qian E-mail: qianjun{at}biozeron.com; Ran Xu E-mail: 53509011{at}qq.com; Baosheng Liao E-mail: swjs0821bs{at}126.com; Liang Shen E-mail: lshen{at}icmm.ac.cn; Mingli Wu E-mail: justuswu{at}163.com; Ruiyang Cheng E-mail: 18612545661{at}163.com

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted May 29, 2017.
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Inoculation of biocontrol bacteria alleviated Panax ginseng replanting problem
Linlin Dong, Jiang Xu, Lianjuan Zhang, Guangfei Wei, He Su, Juan Yang, Jun Qian, Ran Xu, Baosheng Liao, Liang Shen, Mingli Wu, Ruiyang Cheng, Shilin Chen
bioRxiv 143412; doi: https://doi.org/10.1101/143412
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Inoculation of biocontrol bacteria alleviated Panax ginseng replanting problem
Linlin Dong, Jiang Xu, Lianjuan Zhang, Guangfei Wei, He Su, Juan Yang, Jun Qian, Ran Xu, Baosheng Liao, Liang Shen, Mingli Wu, Ruiyang Cheng, Shilin Chen
bioRxiv 143412; doi: https://doi.org/10.1101/143412

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