Abstract
Introduction The control over multi-resistant nosocomial strains of microorganisms has been becoming increasingly urgent in recent years. We suggest a new paradigm that eliminates killing or inhibiting the growth of bacteria. Excluding bacteria death supresses the selection of resistant strains of microorganisms. We have developed such non-metabolite growth promoters, which in very low doses stimulate the rapid growth of many bacteria strains. The mechanism of action of the enhancers is caused by the activation of the cAMP high doses accumulation process in the microbial cells. cAMP itself is a substrate for phosphorylation including DNA polymerases.
Materials and methods The susceptible culture collection resistance strains Pseudomonas aeruginosa MDR Kharkov IMI1, Acinetobacter baumanii MDR Kharkov-IMI1, and Clebsiella pneumonia MDR Kharkov-IMI1 were used. The following antimicrobial agents of known potency were evaluated: ciprofloxacin, polymyxin, amikacin. The same broth, but containing 0.001% enhancers (under patenting), has been used for further passaging for MDR strains. Characteristics of bacterial growth were determined in a medium compared at the control group – the broth without enhancers.
Results and discussion Enhancers contribute to a significant increase in the antimicrobial sensitivity to polymyxin, ciprofloxacin and amikacin in multi-resistant strains of bacteria. Changes in the growth characteristics and antimicrobial sensitivity are observed only in the second passage that demonstrates the need for the further studies of the molecular mechanisms of the cAMP effect on the division and growth of microbial cells.
