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Endocytosis caused by liquid-liquid phase separation of proteins

Louis-Philippe Bergeron-Sandoval, Hossein Khadivi Heris, Catherine Chang, Caitlin E. Cornell, Sarah L. Keller, View ORCID ProfilePaul François, Adam G. Hendricks, Allen J. Ehrlicher, Rohit V. Pappu, Stephen W. Michnick
doi: https://doi.org/10.1101/145664
Louis-Philippe Bergeron-Sandoval
1Département de Biochimie, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, Québec, H3C 3J7, Canada.
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Hossein Khadivi Heris
2Department of Bioengineering, McGill University, 817 Sherbrooke St West, Room 270, Montreal, Quebec, H3A 0C3, Canada.
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Catherine Chang
3Department of Chemistry, University of Washington, Seattle, Seattle WA 98195-1700 USA
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Caitlin E. Cornell
3Department of Chemistry, University of Washington, Seattle, Seattle WA 98195-1700 USA
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Sarah L. Keller
3Department of Chemistry, University of Washington, Seattle, Seattle WA 98195-1700 USA
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Paul François
4Ernest Rutherford Physics Building, McGill University, 3600 University St, Montreal, Québec, H3A 2T8
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  • ORCID record for Paul François
Adam G. Hendricks
2Department of Bioengineering, McGill University, 817 Sherbrooke St West, Room 270, Montreal, Quebec, H3A 0C3, Canada.
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Allen J. Ehrlicher
2Department of Bioengineering, McGill University, 817 Sherbrooke St West, Room 270, Montreal, Quebec, H3A 0C3, Canada.
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Rohit V. Pappu
5Department of Biomedical Engineering and Center for Biological Systems Engineering, Washington University in St. Louis, One Brookings Drive, Campus Box 1097, St. Louis, Missouri 63130, USA.
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Stephen W. Michnick
1Département de Biochimie, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, Québec, H3C 3J7, Canada.
6Centre Robert-Cedergren, Bio-Informatique et Génomique, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, Québec, H3C 3J7, Canada.
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  • For correspondence: stephen.michnick@umontreal.ca
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Summary

Endocytosis underlies intra- and extracellular material trafficking in eukaryotes, and is essential to protein metabolism, intercellular signaling, membrane remodeling and other cell regulatory processes. Although endocytosis is usually driven by F-actin polymerization in yeast cells, membrane invagination can also occur through a yet unknown actin-independent mechanism when turgor pressure is relieved. Here, we demonstrate that membrane invagination can arise from liquid-liquid phase separation (demixing) of proteins with prion-like domains (PLD) from the cytosol. Demixing of these proteins results in the formation of a protein condensate, which, by virtue of its composition and viscoelastic properties, binds to and deforms plasma membrane and cytosol. Demonstration that phase separated condensates can perform mechanical work expands the repertoire of known functions of protein condensates to include the ability to do work at soft interfaces such as between the condensate and the membrane. Similar mechanisms may govern or contribute to other membrane shaping, invagination and budding processes that are involved in cellular material uptake, secretion, and cell shape remodeling.

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Posted December 04, 2018.
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Endocytosis caused by liquid-liquid phase separation of proteins
Louis-Philippe Bergeron-Sandoval, Hossein Khadivi Heris, Catherine Chang, Caitlin E. Cornell, Sarah L. Keller, Paul François, Adam G. Hendricks, Allen J. Ehrlicher, Rohit V. Pappu, Stephen W. Michnick
bioRxiv 145664; doi: https://doi.org/10.1101/145664
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Endocytosis caused by liquid-liquid phase separation of proteins
Louis-Philippe Bergeron-Sandoval, Hossein Khadivi Heris, Catherine Chang, Caitlin E. Cornell, Sarah L. Keller, Paul François, Adam G. Hendricks, Allen J. Ehrlicher, Rohit V. Pappu, Stephen W. Michnick
bioRxiv 145664; doi: https://doi.org/10.1101/145664

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