Abstract
All ales are fermented by various strains of Saccharomyces cerevisiae. However, recent whole-genome sequencing has revealed that most commercially available ale yeasts are highly related and represent a small fraction of the genetic diversity found among S. cerevisiae isolates as a whole. This lack of diversity limits the phenotypic variations between these strains, which translates into a limited number of sensory compounds created during fermentation. Here, we characterized a collection of wild S. cerevisiae, S. kudriavzevii, and S. paradoxus strains for their ability to ferment wort into beer. Although many isolates performed well, S. cerevisiae strain YH166 was the most promising, displaying excellent fermentation kinetics and attenuation, as well as a tropical fruit sensory profile. Use of this strain in multiple styles of beer suggested that it is broadly applicable in the brewing industry. Thus, YH166 is a novel ale strain that can be used to lend fruity esters to beer and should pair well with citrusy hops in hop-forward ales.
Footnotes
klosburn{at}umail.iu.edu; bochman{at}indiana.edu
rob{at}drinkin.beer; justin{at}blackacrebrewing.com
Chemical compounds studied in this article: Ethanol (PubChem CID: 702); glucose (PubChem CID: 5793); maltose (PubChem CID: 6255); sucrose (PubChem CID: 5988); xylose (PubChem CID: 135191)
Abbreviations: EtOH, ethanol; YPD, yeast extract, peptone, and dextrose; NJ, neighbor-joining; OD660, optical density at 660 nm; OG, original gravity; FG, final gravity; ABV, alcohol by volume; NE-IPA, New England-style India pale ale