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Dual role of E-cadherin in the regulation of invasive collective migration of mammary carcinoma cells

Yair Elisha, Vyacheslav Kalchenko, Yuri Kuznetsov, Benjamin Geiger
doi: https://doi.org/10.1101/151613
Yair Elisha
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 7610001, Israel
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Vyacheslav Kalchenko
2Department of Veterinary Resources, Weizmann Institute of Science, Rehovot 7610001, Israel
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Yuri Kuznetsov
2Department of Veterinary Resources, Weizmann Institute of Science, Rehovot 7610001, Israel
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Benjamin Geiger
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 7610001, Israel
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  • For correspondence: benny.geiger@weizmann.ac.il
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Abstract

In this article, we explore a non-canonical form of collective cell migration, displayed by the metastatic murine mammary carcinoma cell line 4T1. We show here that in sparsely plated 4T1 cells, E-cadherin levels are moderately reduced (~50%), leading to the development of collective migration, whereby cells translocate in loose clusters, interconnected by thin membrane tethers. Knocking down E-cadherin blocked tether formation in these cells, leading to enhancement of migration rate and, at the same time, to suppression of lung metastases formation in vivo, and inhibition of infiltration into fibroblast monolayers ex vivo. These findings suggest that the moderate E-cadherin levels, present in wild-type 4T1 cells, play a key role in promoting cancer invasion and metastasis.

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Posted June 18, 2017.
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Dual role of E-cadherin in the regulation of invasive collective migration of mammary carcinoma cells
Yair Elisha, Vyacheslav Kalchenko, Yuri Kuznetsov, Benjamin Geiger
bioRxiv 151613; doi: https://doi.org/10.1101/151613
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Dual role of E-cadherin in the regulation of invasive collective migration of mammary carcinoma cells
Yair Elisha, Vyacheslav Kalchenko, Yuri Kuznetsov, Benjamin Geiger
bioRxiv 151613; doi: https://doi.org/10.1101/151613

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