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Emergent properties in complex synthetic bacterial promoters

Lummy Maria Oliveira Monteiro, Letícia Magalhães Arruda, Rafael Silva-Rocha
doi: https://doi.org/10.1101/152934
Lummy Maria Oliveira Monteiro
Systems and Synthetic Biology Lab, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil
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Letícia Magalhães Arruda
Systems and Synthetic Biology Lab, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil
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Rafael Silva-Rocha
Systems and Synthetic Biology Lab, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil
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  • For correspondence: silvarochar@gmail.com
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Summary

Regulation of gene expression in bacteria results from the interplay between transcriptional factors (TFs) at target promoters, and how the arrangement of binding sites determines the regulatory logic of promoters is not well known. Here, we generated and fully characterized a library of synthetic complex promoters for the global regulators, CRP and IHF, in Escherichia coli, formed by a weak -35/-10 consensus sequence preceded by four combinatorial binding sites for these TFs. We found that while cis-elements for CRP preferentially activate promoters when located immediately upstream of the promoter consensus, binding sites for IHF mainly function as “UP” elements and stimulate transcription in several different architectures in the absence of this protein. However, the combination of CRP- and IHF-binding sites resulted in emergent properties in these complex promoters, where the activity of combinatorial promoters cannot be predicted from the individual behavior of its components. Taken together, the results presented here add to the information on architecture-logic of complex promoters in bacteria.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 20, 2017.
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Emergent properties in complex synthetic bacterial promoters
Lummy Maria Oliveira Monteiro, Letícia Magalhães Arruda, Rafael Silva-Rocha
bioRxiv 152934; doi: https://doi.org/10.1101/152934
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Emergent properties in complex synthetic bacterial promoters
Lummy Maria Oliveira Monteiro, Letícia Magalhães Arruda, Rafael Silva-Rocha
bioRxiv 152934; doi: https://doi.org/10.1101/152934

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