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A method to minimize condenser lens-induced hysteresis effects in a JEOL JEM-3200FSC microscope to enable stable cryoEM low-dose operations

M. Jason de la Cruz, Michael Martynowycz, Johan Hattne, Dan Shi, Tamir Gonen
doi: https://doi.org/10.1101/153395
M. Jason de la Cruz
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn VA 20147, USA.
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Michael Martynowycz
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn VA 20147, USA.
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Johan Hattne
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn VA 20147, USA.
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Dan Shi
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn VA 20147, USA.
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Tamir Gonen
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn VA 20147, USA.
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  • For correspondence: gonent@janelia.hhmi.org
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Abstract

Low dose imaging procedures are key for a successful cryoEM experiment (whether by electron cryotomography, single particle analysis, electron crystallography, or MicroED). We present a method to minimize magnetic hysteresis of the condenser lens system in the JEOL JEM-3200FSC transmission electron microscope (TEM) in order to maintain a stable optical axis for the beam path of low-dose imaging. The simple procedure involves independent voltage ramping of the CL1 and CL2 lenses immediately before switching to the focusing and exposure beam settings for data collection.

Highlights

  • Enables consistent horizontal beam position during low-dose mode switching

  • Facilitates EM automated data collection for the JEOL JEM-3200FSC

  • Improves beam stability in both TEM imaging and diffraction modes

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted June 21, 2017.
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A method to minimize condenser lens-induced hysteresis effects in a JEOL JEM-3200FSC microscope to enable stable cryoEM low-dose operations
M. Jason de la Cruz, Michael Martynowycz, Johan Hattne, Dan Shi, Tamir Gonen
bioRxiv 153395; doi: https://doi.org/10.1101/153395
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A method to minimize condenser lens-induced hysteresis effects in a JEOL JEM-3200FSC microscope to enable stable cryoEM low-dose operations
M. Jason de la Cruz, Michael Martynowycz, Johan Hattne, Dan Shi, Tamir Gonen
bioRxiv 153395; doi: https://doi.org/10.1101/153395

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