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Corrigendum and follow-up: Whole genome sequencing of multiple CRISPR-edited mouse lines suggests no excess mutations

Kellie A. Schaefer, Benjamin W Darbro, Diana F. Colgan, Stephen H. Tsang, Alexander G. Bassuk, Vinit B. Mahajan
doi: https://doi.org/10.1101/154450
Kellie A. Schaefer
1Omics Laboratory, Stanford University, Palo Alto, CA
2Interdisciplinary Graduate Program in Genetics, University of Iowa, Iowa City, IA
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Benjamin W Darbro
3Department of Pediatrics, University of Iowa, Iowa City, IA
M.D., PhD.
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Diana F. Colgan
1Omics Laboratory, Stanford University, Palo Alto, CA
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Stephen H. Tsang
4Jonas Children’s Vision Care, and Bernard & Shirlee Brown Glaucoma Laboratory, Departments of Ophthalmology, Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, New York, NY 10032
5Edward S. Harkness Eye Institute, New York-Presbyterian Hospital, New York, NY 10032
M.D., Ph.D.
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Alexander G. Bassuk
3Department of Pediatrics, University of Iowa, Iowa City, IA
6Neurology, University of Iowa, Iowa City, IA
M.D., Ph.D.
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  • For correspondence: vinit.mahajan@stanford.edu alexander-bassuk@uiowa.edu
Vinit B. Mahajan
1Omics Laboratory, Stanford University, Palo Alto, CA
7Byers Eye Institute, Stanford University, Palo Alto, CA
M.D. Ph.D.
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  • For correspondence: vinit.mahajan@stanford.edu alexander-bassuk@uiowa.edu
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Abstract

Our previous publication suggested CRISPR-Cas9 editing at the zygotic stage might unexpectedly introduce a multitude of subtle but unintended mutations, an interpretation that not surprisingly raised numerous questions. The key issue is that since parental lines were not available, might the reported variants have been inherited? To expand upon the limited available whole genome data on whether CRISPR-edited mice show more genetic variation, whole-genome sequencing was performed on two other mouse lines that had undergone a CRISPR-editing procedure. Again, parents were not available for either the Capn5 nor Fblim1 CRISPR-edited mouse lines, so strain controls were examined. Additionally, we also include verification of variants detected in the initial mouse line. Taken together, these whole-genome-sequencing-level results support the idea that in specific cases, CRISPR-Cas9 editing can precisely edit the genome at the organismal level and may not introduce numerous, unintended, off-target mutations.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 26, 2018.
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Corrigendum and follow-up: Whole genome sequencing of multiple CRISPR-edited mouse lines suggests no excess mutations
Kellie A. Schaefer, Benjamin W Darbro, Diana F. Colgan, Stephen H. Tsang, Alexander G. Bassuk, Vinit B. Mahajan
bioRxiv 154450; doi: https://doi.org/10.1101/154450
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Corrigendum and follow-up: Whole genome sequencing of multiple CRISPR-edited mouse lines suggests no excess mutations
Kellie A. Schaefer, Benjamin W Darbro, Diana F. Colgan, Stephen H. Tsang, Alexander G. Bassuk, Vinit B. Mahajan
bioRxiv 154450; doi: https://doi.org/10.1101/154450

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