Abstract
We recently developed a RNA-binding protein target identification tool, TRIBE, which expresses an RBP of interest fused to the catalytic domain (cd) of the RNA editing enzyme ADAR. Here we describe HyperTRIBE, which carries a hyperactive mutation (E488Q) of the ADAR catalytic domain. HyperTRIBE identifies dramatically more targets and more faithfully recapitulates the known binding specificity of its RBP than TRIBE.
Copyright
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