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Three-dimensional cell neighbourhood impacts differentiation in the inner mass cells of the mouse blastocyst

Sabine C. Fischer, Elena Corujo-Simón, Joaquín Lilao-Garzón, View ORCID ProfileErnst H. K. Stelzer, View ORCID ProfileSilvia Muñoz-Descalzo
doi: https://doi.org/10.1101/159301
Sabine C. Fischer
1Physikalische Biologie (IZN, FB15), Buchmann Institute for Molecular Life Sciences (BMLS), Goethe-Universität Frankfurt am Main (Germany).
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Elena Corujo-Simón
2Dept. of Biology and Biochemistry, University of Bath (United Kingdom).
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Joaquín Lilao-Garzón
2Dept. of Biology and Biochemistry, University of Bath (United Kingdom).
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Ernst H. K. Stelzer
1Physikalische Biologie (IZN, FB15), Buchmann Institute for Molecular Life Sciences (BMLS), Goethe-Universität Frankfurt am Main (Germany).
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  • ORCID record for Ernst H. K. Stelzer
  • For correspondence: ernst.stelzer@physikalischebiologie.de s.munoz-descalzo@bath.ac.uk
Silvia Muñoz-Descalzo
2Dept. of Biology and Biochemistry, University of Bath (United Kingdom).
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  • For correspondence: ernst.stelzer@physikalischebiologie.de s.munoz-descalzo@bath.ac.uk
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Abstract

During mammalian blastocyst development, inner cell mass (ICM) cells differentiate into epiblast (Epi) or primitive endoderm (PrE). These two fates are characterised by the transcription factors NANOG and GATA6, respectively. Here, we present quantitative three-dimensional single cell-based neighbourhood analyses to investigate the spatial distribution of NANOG and GATA6 expression in the ICM of the mouse blastocyst. The cell neighbourhood is characterised by the expression levels of the fate markers in the surrounding cells, together with the number of surrounding cells and cell position. We find that cell neighbourhoods are established in early blastocysts and different for cells expressing different levels of NANOG and GATA6. Highest NANOG expressing cells occupy specific positions within the ICM and are surrounded by 9 neighbours, while GATA6 expressing cells cluster according to their GATA6 levels. The analysis of mutants reveals that NANOG local neighbourhood is regulated by GATA6.

Summary statement Three-dimensional cell neighbourhood, which includes fate marker levels, number of neighbouring cells and cell position, determines cell fate decision in early mouse embryos.

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Posted July 04, 2017.
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Three-dimensional cell neighbourhood impacts differentiation in the inner mass cells of the mouse blastocyst
Sabine C. Fischer, Elena Corujo-Simón, Joaquín Lilao-Garzón, Ernst H. K. Stelzer, Silvia Muñoz-Descalzo
bioRxiv 159301; doi: https://doi.org/10.1101/159301
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Three-dimensional cell neighbourhood impacts differentiation in the inner mass cells of the mouse blastocyst
Sabine C. Fischer, Elena Corujo-Simón, Joaquín Lilao-Garzón, Ernst H. K. Stelzer, Silvia Muñoz-Descalzo
bioRxiv 159301; doi: https://doi.org/10.1101/159301

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