Contradictory Results
“Unexpected mutations after CRISPR-Cas9 editing in vivo” are most likely pre-existing sequence variants and not nuclease-induced mutations
Caleb A. Lareau, Kendell Clement, Jonathan Y. Hsu, Vikram Pattanayak, J. Keith Joung, View ORCID ProfileMartin J. Aryee, Luca Pinello
doi: https://doi.org/10.1101/159707
Caleb A. Lareau
1Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA 02129 USA
2Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA 02115 USA
3Cell Circuits and Epigenomics Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142 USA
Kendell Clement
1Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA 02129 USA
3Cell Circuits and Epigenomics Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142 USA
4Department of Pathology, Harvard Medical School, Boston, MA 02115
5Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138 USA
Jonathan Y. Hsu
1Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA 02129 USA
6Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 USA
Vikram Pattanayak
1Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA 02129 USA
4Department of Pathology, Harvard Medical School, Boston, MA 02115
J. Keith Joung
1Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA 02129 USA
4Department of Pathology, Harvard Medical School, Boston, MA 02115
Martin J. Aryee
1Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA 02129 USA
2Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA 02115 USA
3Cell Circuits and Epigenomics Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142 USA
4Department of Pathology, Harvard Medical School, Boston, MA 02115
Luca Pinello
1Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA 02129 USA
4Department of Pathology, Harvard Medical School, Boston, MA 02115
Article usage
Posted July 05, 2017.
“Unexpected mutations after CRISPR-Cas9 editing in vivo” are most likely pre-existing sequence variants and not nuclease-induced mutations
Caleb A. Lareau, Kendell Clement, Jonathan Y. Hsu, Vikram Pattanayak, J. Keith Joung, Martin J. Aryee, Luca Pinello
bioRxiv 159707; doi: https://doi.org/10.1101/159707
“Unexpected mutations after CRISPR-Cas9 editing in vivo” are most likely pre-existing sequence variants and not nuclease-induced mutations
Caleb A. Lareau, Kendell Clement, Jonathan Y. Hsu, Vikram Pattanayak, J. Keith Joung, Martin J. Aryee, Luca Pinello
bioRxiv 159707; doi: https://doi.org/10.1101/159707
Subject Area
Subject Areas
- Biochemistry (13135)
- Bioengineering (9972)
- Bioinformatics (31969)
- Biophysics (16500)
- Cancer Biology (13567)
- Cell Biology (19283)
- Clinical Trials (138)
- Developmental Biology (10432)
- Ecology (15483)
- Epidemiology (2067)
- Evolutionary Biology (19763)
- Genetics (13086)
- Genomics (18114)
- Immunology (13225)
- Microbiology (30954)
- Molecular Biology (12887)
- Neuroscience (67587)
- Paleontology (495)
- Pathology (2081)
- Pharmacology and Toxicology (3600)
- Physiology (5620)
- Plant Biology (11576)
- Synthetic Biology (3233)
- Systems Biology (7915)
- Zoology (1792)