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Automated Parameterization of Predictive Kinetic Metabolic Models from Sparse Datasets for Efficient Optimization of Many-Enzyme Heterologous Pathways

Sean M. Halper, View ORCID ProfileIman Farasat, View ORCID ProfileHoward M. Salis
doi: https://doi.org/10.1101/161372
Sean M. Halper
Department of Chemical Engineering, Pennsylvania State University, University Park, PA 16802
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Iman Farasat
Department of Chemical Engineering, Pennsylvania State University, University Park, PA 16802
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Howard M. Salis
Department of Chemical Engineering, Pennsylvania State University, University Park, PA 16802Department of Biological Engineering, Pennsylvania State University, University Park, PA 16802
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  • ORCID record for Howard M. Salis
  • For correspondence: salis@psu.edu
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Abstract

Engineered heterologous metabolic pathways can convert low-cost feedstock into high-value products, though it remains a significant challenge to reliably and efficiently maximize end-product biosynthesis, particularly when many enzymes must be co-expressed together. When current approaches are applied to many-enzyme pathways, the construction and characterization process is highly iterative and laborious, while generating high-dimensional datasets that remain difficult to analyze for forward engineering efforts. To overcome these challenges, we developed a new algorithm that determines the highly non-linear and high-dimensional relationship between a pathway’s enzyme expression levels and its end-product productivity from common characterization of a small number of heterologous pathway variants. We combined kinetic metabolic modeling, elementary mode analysis, model reduction, de-dimensionalization, and genetic algorithm optimization into an automated procedure that parameterizes accurate kinetic metabolic models from sparsely characterized pathway variant libraries with varied enzyme expression levels. The resulting Pathway Maps are used to determine rate-limiting steps, predict optimal expression levels, identify allosteric interactions, rank-order enzyme kinetics, and prioritize protein engineering efforts. We demonstrate the Pathway Map Calculator algorithm on two experimental datasets, a 3-enzyme carotenoid biosynthesis pathway and a 9-enzyme limonene biosynthesis pathway, as well as a series of in silico pathway examples to rigorously demonstrate the algorithm’s accuracy, linear scaling, and high tolerance to measurement noise. By greatly reducing experimental efforts and providing quantitative forward engineering predictions, the Pathway Map Calculator has the potential to dramatically accelerate the engineering of many-enzyme heterologous metabolic pathways.

Highlights

  • We developed an automated algorithm that uses a small number of characterized pathway variants to determine the pathway’s expression-productivity relationship.

  • The Pathway Map Calculator is accurate, scales linearly on many-enzyme pathways, distinguishes allosteric interactions, and tolerates substantial measurement noise.

  • Pathway Maps are used to predict optimal enzyme expression levels, identify rate-limiting steps, and prioritize protein engineering efforts

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted July 09, 2017.
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Automated Parameterization of Predictive Kinetic Metabolic Models from Sparse Datasets for Efficient Optimization of Many-Enzyme Heterologous Pathways
Sean M. Halper, Iman Farasat, Howard M. Salis
bioRxiv 161372; doi: https://doi.org/10.1101/161372
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Automated Parameterization of Predictive Kinetic Metabolic Models from Sparse Datasets for Efficient Optimization of Many-Enzyme Heterologous Pathways
Sean M. Halper, Iman Farasat, Howard M. Salis
bioRxiv 161372; doi: https://doi.org/10.1101/161372

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