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GUIDES: sgRNA design for loss-of-function screens

Joshua A. Meier, Feng Zhang, Neville E. Sanjana
doi: https://doi.org/10.1101/168849
Joshua A. Meier
1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
2McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge MA 02139, USA
3New York Genome Center, 101 Avenue of the Americas, New York, NY 10013
4Department of Biology, New York University, 100 Washington Square East, New York, NY 10003
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Feng Zhang
1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
2McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge MA 02139, USA
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Neville E. Sanjana
3New York Genome Center, 101 Avenue of the Americas, New York, NY 10013
4Department of Biology, New York University, 100 Washington Square East, New York, NY 10003
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  • For correspondence: neville@sanjanalab.org
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Abstract

GUIDES (Graphical User Interface for DNA Editing Screens) is a web-based tool for the design of custom, large-scale CRISPR libraries for loss-of-function screens in human and mouse. GUIDES combines multi-tissue RNA-sequencing data to target expressed exons, protein annotation to target functional domains, sophisticated on-target and off-target guide RNA scoring and other optimizations to create CRISPR libraries directly from a list of genes without requiring any programming expertise.

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Posted July 26, 2017.
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GUIDES: sgRNA design for loss-of-function screens
Joshua A. Meier, Feng Zhang, Neville E. Sanjana
bioRxiv 168849; doi: https://doi.org/10.1101/168849
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GUIDES: sgRNA design for loss-of-function screens
Joshua A. Meier, Feng Zhang, Neville E. Sanjana
bioRxiv 168849; doi: https://doi.org/10.1101/168849

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