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In situ genome editing method suitable for routine generation of germline modified animal models

Masato Ohtsuka, Hiromi Miura, Naomi Arifin, Shingo Nakamura, Kenta Wada, Channabasavaiah B. Gurumurthy, Masahiro Sato
doi: https://doi.org/10.1101/172718
Masato Ohtsuka
1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, Japan
3The Institute of Medical Sciences, Tokai University, Isehara, Kanagawa, Japan
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Hiromi Miura
1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, Japan
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Naomi Arifin
1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
4Department of Applied Biochemistry, School of Engineering, Tokai University, Hiratsuka, Kanagawa, Japan
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Shingo Nakamura
5Division of Biomedical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan
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Kenta Wada
6Department of Bioproduction, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan
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Channabasavaiah B. Gurumurthy
7Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA
8Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA
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Masahiro Sato
9Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Kagoshima, Japan
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Article Information

doi 
https://doi.org/10.1101/172718
History 
  • August 4, 2017.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Masato Ohtsuka1,2,3,
  2. Hiromi Miura1,2,
  3. Naomi Arifin1,4,
  4. Shingo Nakamura5,
  5. Kenta Wada6,
  6. Channabasavaiah B. Gurumurthy7,8 and
  7. Masahiro Sato9
  1. 1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
  2. 2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, Japan
  3. 3The Institute of Medical Sciences, Tokai University, Isehara, Kanagawa, Japan
  4. 4Department of Applied Biochemistry, School of Engineering, Tokai University, Hiratsuka, Kanagawa, Japan
  5. 5Division of Biomedical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan
  6. 6Department of Bioproduction, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan
  7. 7Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA
  8. 8Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA
  9. 9Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Kagoshima, Japan
  1. Corresponding author: Masato Ohtsuka (masato{at}is.icc.u-tokai.ac.jp) Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Kanagawa 259-1193, Japan
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Posted August 04, 2017.
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In situ genome editing method suitable for routine generation of germline modified animal models
Masato Ohtsuka, Hiromi Miura, Naomi Arifin, Shingo Nakamura, Kenta Wada, Channabasavaiah B. Gurumurthy, Masahiro Sato
bioRxiv 172718; doi: https://doi.org/10.1101/172718
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In situ genome editing method suitable for routine generation of germline modified animal models
Masato Ohtsuka, Hiromi Miura, Naomi Arifin, Shingo Nakamura, Kenta Wada, Channabasavaiah B. Gurumurthy, Masahiro Sato
bioRxiv 172718; doi: https://doi.org/10.1101/172718

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