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In situ genome editing method suitable for routine generation of germline modified animal models

Masato Ohtsuka, Hiromi Miura, Naomi Arifin, Shingo Nakamura, Kenta Wada, Channabasavaiah B. Gurumurthy, Masahiro Sato
doi: https://doi.org/10.1101/172718
Masato Ohtsuka
1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, Japan
3The Institute of Medical Sciences, Tokai University, Isehara, Kanagawa, Japan
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Hiromi Miura
1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, Japan
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Naomi Arifin
1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
4Department of Applied Biochemistry, School of Engineering, Tokai University, Hiratsuka, Kanagawa, Japan
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Shingo Nakamura
5Division of Biomedical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan
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Kenta Wada
6Department of Bioproduction, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan
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Channabasavaiah B. Gurumurthy
7Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA
8Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA
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Masahiro Sato
9Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Kagoshima, Japan
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Posted August 04, 2017.
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In situ genome editing method suitable for routine generation of germline modified animal models
Masato Ohtsuka, Hiromi Miura, Naomi Arifin, Shingo Nakamura, Kenta Wada, Channabasavaiah B. Gurumurthy, Masahiro Sato
bioRxiv 172718; doi: https://doi.org/10.1101/172718
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In situ genome editing method suitable for routine generation of germline modified animal models
Masato Ohtsuka, Hiromi Miura, Naomi Arifin, Shingo Nakamura, Kenta Wada, Channabasavaiah B. Gurumurthy, Masahiro Sato
bioRxiv 172718; doi: https://doi.org/10.1101/172718

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