In situ genome editing method suitable for routine generation of germline modified animal models

Masato Ohtsuka; Hiromi Miura; Naomi Arifin; Shingo Nakamura; Kenta Wada; Channabasavaiah B. Gurumurthy; Masahiro Sato

doi:10.1101/172718

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In situ genome editing method suitable for routine generation of germline modified animal models

Masato Ohtsuka, Hiromi Miura, Naomi Arifin, Shingo Nakamura, Kenta Wada, Channabasavaiah B. Gurumurthy, Masahiro Sato

doi: https://doi.org/10.1101/172718

Masato Ohtsuka

1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan

2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, Japan

3The Institute of Medical Sciences, Tokai University, Isehara, Kanagawa, Japan

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Hiromi Miura

1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan

2Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, Japan

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Naomi Arifin

1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan

4Department of Applied Biochemistry, School of Engineering, Tokai University, Hiratsuka, Kanagawa, Japan

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Shingo Nakamura

5Division of Biomedical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan

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Kenta Wada

6Department of Bioproduction, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan

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Channabasavaiah B. Gurumurthy

7Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA

8Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA

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Masahiro Sato

9Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Kagoshima, Japan

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In situ genome editing method suitable for routine generation of germline modified animal models

Masato Ohtsuka, Hiromi Miura, Naomi Arifin, Shingo Nakamura, Kenta Wada, Channabasavaiah B. Gurumurthy, Masahiro Sato

bioRxiv 172718; doi: https://doi.org/10.1101/172718

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In situ genome editing method suitable for routine generation of germline modified animal models

Masato Ohtsuka, Hiromi Miura, Naomi Arifin, Shingo Nakamura, Kenta Wada, Channabasavaiah B. Gurumurthy, Masahiro Sato

bioRxiv 172718; doi: https://doi.org/10.1101/172718

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