Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Induction of X-chromosome Inactivation by the Histone Demethylase SMCX/KDM5C

Srimonta Gayen, Emily Maclary, Yumie Murata-Nakamura, Christina N. Vallianatos, Robert S. Porter, Patricia M. Garay, Shigeki Iwase, View ORCID ProfileSundeep Kalantry
doi: https://doi.org/10.1101/175174
Srimonta Gayen
1Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-5618
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Emily Maclary
1Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-5618
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yumie Murata-Nakamura
1Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-5618
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christina N. Vallianatos
1Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-5618
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robert S. Porter
1Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-5618
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Patricia M. Garay
1Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-5618
2Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109-5618
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shigeki Iwase
1Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-5618
2Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109-5618
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: kalantry@umich.edu siwase@umich.edu
Sundeep Kalantry
1Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-5618
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sundeep Kalantry
  • For correspondence: kalantry@umich.edu siwase@umich.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

SUMMARY

XY male and XX female mammals equalize X-linked gene expression through the mitotically-stable transcriptional inactivation of an X-chromosome in females. Although most genes are silent on the inactive-X, some escape silencing and are expressed at higher levels in females vs. males. Here, we show that the escapee Smcx/Kdm5c, encoding a histone H3K4me2/3 demethylase, underlies the female-specific induction of X-inactivation. Mouse embryonic epiblast cells and differentiating embryonic stem cells (ESCs) lacking SMCX show reduced expression of Xist RNA, which is required for X-inactivation. Smcx-heterozygous epiblast cells do not silence X-linked genes efficiently, despite robust Xist expression. Overexpression of mouse or human SMCX, but not a catalytically-inactive SMCX or the Y-chromosome homolog SMCY, is sufficient to induce Xist and, separately, to silence X-linked genes in male ESCs. Finally, SMCX dose is inversely correlated with H3K4me2 at X-linked loci. Thus, X-inactivation initiates through the evolutionarily conserved, dose-dependent function of the histone demethylase SMCX.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted August 11, 2017.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Induction of X-chromosome Inactivation by the Histone Demethylase SMCX/KDM5C
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Induction of X-chromosome Inactivation by the Histone Demethylase SMCX/KDM5C
Srimonta Gayen, Emily Maclary, Yumie Murata-Nakamura, Christina N. Vallianatos, Robert S. Porter, Patricia M. Garay, Shigeki Iwase, Sundeep Kalantry
bioRxiv 175174; doi: https://doi.org/10.1101/175174
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Induction of X-chromosome Inactivation by the Histone Demethylase SMCX/KDM5C
Srimonta Gayen, Emily Maclary, Yumie Murata-Nakamura, Christina N. Vallianatos, Robert S. Porter, Patricia M. Garay, Shigeki Iwase, Sundeep Kalantry
bioRxiv 175174; doi: https://doi.org/10.1101/175174

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genetics
Subject Areas
All Articles
  • Animal Behavior and Cognition (2635)
  • Biochemistry (5224)
  • Bioengineering (3650)
  • Bioinformatics (15730)
  • Biophysics (7218)
  • Cancer Biology (5597)
  • Cell Biology (8051)
  • Clinical Trials (138)
  • Developmental Biology (4739)
  • Ecology (7472)
  • Epidemiology (2059)
  • Evolutionary Biology (10531)
  • Genetics (7704)
  • Genomics (10090)
  • Immunology (5157)
  • Microbiology (13829)
  • Molecular Biology (5354)
  • Neuroscience (30605)
  • Paleontology (212)
  • Pathology (872)
  • Pharmacology and Toxicology (1520)
  • Physiology (2235)
  • Plant Biology (4987)
  • Scientific Communication and Education (1037)
  • Synthetic Biology (1380)
  • Systems Biology (4131)
  • Zoology (804)