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Transient Reduction of DNA Methylation at the Onset of Meiosis in Male Mice

Valeriya Gaysinskaya, Brendan F. Miller, Godfried W. van der Heijden, View ORCID ProfileKasper D. Hansen, View ORCID ProfileAlex Bortvin
doi: https://doi.org/10.1101/177535
Valeriya Gaysinskaya
1Department of Embryology, Carnegie Institution for Science, Baltimore, Maryland, United States of America
2Department of Biology, Johns Hopkins University, Baltimore, Maryland, United States of America
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Brendan F. Miller
2Department of Biology, Johns Hopkins University, Baltimore, Maryland, United States of America
3Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America
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Godfried W. van der Heijden
4Department of Obstetrics and Gynaecology, Erasmus MC, University Medical Center, PO BOX 2040, 3000 CA, Rotterdam, The Netherlands
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Kasper D. Hansen
5Department of Biostatistics, Johns Hopkins University, Baltimore, Maryland, United States of America
6Center for Computational Biology, Johns Hopkins University, Baltimore, Maryland, United States of America
7McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Maryland, United States of America
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Alex Bortvin
1Department of Embryology, Carnegie Institution for Science, Baltimore, Maryland, United States of America
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Abstract

The quality of germ cells depends on successful chromatin organization in meiotic prophase I (MPI). To better understand the epigenetic context of MPI we studied the dynamics of DNA methylation in wild-type male mice. We discovered an extended period of genome-wide transient reduction of DNA methylation (TRDM) during early MPI. Our data show that TRDM arises by passive demethylation in the premeiotic S phase highlighting the abundance of hemimethylated DNA in MPI. Importantly, TRDM unmasks a deficit in retrotransposon LINE-1 DNA methylation contributing to its expression in early MPI. We propose that TRDM facilitates meiosis and gamete quality control.

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Posted August 17, 2017.
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Transient Reduction of DNA Methylation at the Onset of Meiosis in Male Mice
Valeriya Gaysinskaya, Brendan F. Miller, Godfried W. van der Heijden, Kasper D. Hansen, Alex Bortvin
bioRxiv 177535; doi: https://doi.org/10.1101/177535
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Transient Reduction of DNA Methylation at the Onset of Meiosis in Male Mice
Valeriya Gaysinskaya, Brendan F. Miller, Godfried W. van der Heijden, Kasper D. Hansen, Alex Bortvin
bioRxiv 177535; doi: https://doi.org/10.1101/177535

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