Abstract
Across all domains of life, elaborate control mechanisms regulate proteins, pathways, and cell phenotypes as organisms adapt to ever-changing environments. Post-translational modifications (PTMs) allow cells to rapidly and reversibly regulate molecular pathways, but it remains unclear how individual PTMs regulate fitness. Here, we studied >130 PTM sites in Escherichia coli to unravel how PTMs regulate cell metabolism and fitness in response to environmental changes, such as the glucose-acetate diauxie. Using a new metabolic modeling approach, we found a significant fraction of post-translationally modified enzymes are predicted to control shifts in pathway usage following evolutionarily-important environmental changes. Genetic screens using Multiplex Automated Genome Engineering confirmed that these PTMs impact cellular fitness, especially under dynamically changing environments. Finally, mechanisms of how individual PTMs impact protein function were detailed using molecular dynamics simulations and enzyme assays for enolase, transaldolase, and serine hydroxymethyltransferase. Thus, by integrating whole-cell data and pathway modeling with detailed biochemical analysis, we unraveled how individual PTMs regulate enzymes, pathways, and phenotypes to adapt to sudden environmental changes.