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PrimerServer: a high-throughput primer design and specificity-checking platform

Tao Zhu, Chengzhen Liang, Zhigang Meng, Yanyan Li, Yayu Wu, Sandui Guo, Rui Zhang
doi: https://doi.org/10.1101/181941
Tao Zhu
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, China
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Chengzhen Liang
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, China
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Zhigang Meng
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, China
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Yanyan Li
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, China
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Yayu Wu
2College of Biology, Hunan University, 410082, Changsha, China
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Sandui Guo
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, China
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  • For correspondence: zhangrui@caas.cn guosandui@caas.cn
Rui Zhang
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, China
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  • For correspondence: zhangrui@caas.cn guosandui@caas.cn
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Abstract

Summary Designing specific primers for multiple sites across the whole genome is still challenging, especially in species with complex genomes. Here we present PrimerServer, a high-throughput primer design and specificity-checking platform with both web and command-line interfaces. This platform efficiently integrates site selection, primer design, specificity checking and data presentation. In our case study, PrimerServer achieved high accuracy and a fast running speed for a large number of sites, suggesting its potential for molecular biology applications such as molecular breeding or medical testing.

Availability and Implementation Source code for PrimerServer is available at https://github.com/billzt/PrimerServer. A demo server is freely accessible at https://primerserver.org, with all major browsers supported.

Contact zhangrui{at}caas.cn or guosandui{at}caas.cn

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted August 30, 2017.
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PrimerServer: a high-throughput primer design and specificity-checking platform
Tao Zhu, Chengzhen Liang, Zhigang Meng, Yanyan Li, Yayu Wu, Sandui Guo, Rui Zhang
bioRxiv 181941; doi: https://doi.org/10.1101/181941
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PrimerServer: a high-throughput primer design and specificity-checking platform
Tao Zhu, Chengzhen Liang, Zhigang Meng, Yanyan Li, Yayu Wu, Sandui Guo, Rui Zhang
bioRxiv 181941; doi: https://doi.org/10.1101/181941

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