Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Cryo-EM shows how dynactin recruits two dyneins for faster movement

Linas Urnavicius, Clinton K. Lau, Mohamed M. Elshenawy, Edgar Morales-Rios, Carina Motz, Ahmet Yildiz, Andrew P. Carter
doi: https://doi.org/10.1101/183160
Linas Urnavicius
1Medical Research Council Laboratory of Molecular Biology, Division of Structural Studies, Francis Crick Avenue, Cambridge CB2 0QH, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Clinton K. Lau
1Medical Research Council Laboratory of Molecular Biology, Division of Structural Studies, Francis Crick Avenue, Cambridge CB2 0QH, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mohamed M. Elshenawy
2Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Edgar Morales-Rios
3Department of Biochemistry, CINVESTAV, México D.F., Mexico.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carina Motz
1Medical Research Council Laboratory of Molecular Biology, Division of Structural Studies, Francis Crick Avenue, Cambridge CB2 0QH, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ahmet Yildiz
2Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA.
4Physics Department, University of California at Berkeley, Berkeley, California 94720, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrew P. Carter
1Medical Research Council Laboratory of Molecular Biology, Division of Structural Studies, Francis Crick Avenue, Cambridge CB2 0QH, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: cartera@mrc-lmb.cam.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Dynein and its cofactor dynactin form a highly processive microtubule motor in the presence of an activating adaptor, such as BICD2. Different adaptors link dynein/dynactin to distinct cargos. Here we use electron microscopy (EM) and single molecule studies to show that adaptors can recruit a second dynein to dynactin. Whereas BICD2 is biased toward recruiting a single dynein, the adaptors BICDR1 and HOOK3 predominantly recruit two. We find that the shift toward a double dynein complex increases both force and speed. A 3.5 Å cryo-EM reconstruction of a dynein tail/dynactin/BICDR1 complex reveals how dynactin can act as a scaffold to coordinate two dyneins side by side. Our work provides a structural basis for how diverse adaptors recruit different numbers of dyneins and regulate the motile properties of the dynein/dynactin transport machine.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted August 31, 2017.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Cryo-EM shows how dynactin recruits two dyneins for faster movement
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Cryo-EM shows how dynactin recruits two dyneins for faster movement
Linas Urnavicius, Clinton K. Lau, Mohamed M. Elshenawy, Edgar Morales-Rios, Carina Motz, Ahmet Yildiz, Andrew P. Carter
bioRxiv 183160; doi: https://doi.org/10.1101/183160
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Cryo-EM shows how dynactin recruits two dyneins for faster movement
Linas Urnavicius, Clinton K. Lau, Mohamed M. Elshenawy, Edgar Morales-Rios, Carina Motz, Ahmet Yildiz, Andrew P. Carter
bioRxiv 183160; doi: https://doi.org/10.1101/183160

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Molecular Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3512)
  • Biochemistry (7352)
  • Bioengineering (5329)
  • Bioinformatics (20277)
  • Biophysics (10026)
  • Cancer Biology (7749)
  • Cell Biology (11319)
  • Clinical Trials (138)
  • Developmental Biology (6440)
  • Ecology (9958)
  • Epidemiology (2065)
  • Evolutionary Biology (13336)
  • Genetics (9362)
  • Genomics (12592)
  • Immunology (7714)
  • Microbiology (19046)
  • Molecular Biology (7447)
  • Neuroscience (41063)
  • Paleontology (300)
  • Pathology (1231)
  • Pharmacology and Toxicology (2139)
  • Physiology (3164)
  • Plant Biology (6866)
  • Scientific Communication and Education (1274)
  • Synthetic Biology (1898)
  • Systems Biology (5318)
  • Zoology (1089)