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An automated confocal micro-extensometer enables in vivo quantification of mechanical properties with cellular resolution

Sarah Robinson, Michal Huflejt, View ORCID ProfilePierre Barbier de Reuille, View ORCID ProfileSiobhan A. Braybrook, View ORCID ProfileMartine Schorderet, View ORCID ProfileDidier Reinhardt, View ORCID ProfileCris Kuhlemeier
doi: https://doi.org/10.1101/183533
Sarah Robinson
1Institute of Plant Sciences, University of Bern, Bern, Switzerland
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Michal Huflejt
1Institute of Plant Sciences, University of Bern, Bern, Switzerland
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Pierre Barbier de Reuille
1Institute of Plant Sciences, University of Bern, Bern, Switzerland
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Siobhan A. Braybrook
2Sainsbury Laboratory, University of Cambridge, Cambridge, UK
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Martine Schorderet
3Department of Biology, University of Fribourg, Fribourg, Switzerland
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Didier Reinhardt
3Department of Biology, University of Fribourg, Fribourg, Switzerland
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Cris Kuhlemeier
1Institute of Plant Sciences, University of Bern, Bern, Switzerland
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Abstract

How complex developmental-genetic networks are translated into organs with specific 3D shapes remains an open question. This question is particularly challenging because the elaboration of specific shapes is in essence a question of mechanics. In plants, this means how the genetic circuitry affects the cell wall. The mechanical properties of the wall and their spatial variation are the key factors controlling morphogenesis in plants. However, these properties are difficult to measure and investigating their relation to genetic regulation is particularly challenging. To measure spatial variation of mechanical properties, one must determine the deformation of a tissue in response to a known force with cellular resolution. Here we present an automated confocal micro-extensometer (ACME), which greatly expands the scope of existing methods for measuring mechanical properties. Unlike classical extensometers, ACME is mounted on a confocal microscope and utilizes confocal images to compute the deformation of the tissue directly from biological markers, thus providing cellular scale information and improved accuracy. ACME is suitable for measuring the mechanical responses in live tissue. As a proof of concept we demonstrate that the plant hormone gibberellic acid induces a spatial gradient in mechanical properties along the length of the Arabidopsis hypocotyl.

Stress
is the force acting on the material per unit area.
Strain
the relative increase in length of the material, and can be expressed as a percentage change in length.
Mechanical properties
describe the stress-strain relationship for a material. If we apply the same force to a material that is twice as thick/stiff? it will deform half as much, if the material is otherwise the same.
Elastic
elastic materials deform instantly and reversibly.
Creep
a time-dependent irreversible strain that occurs when a constant force is applied and maintained. Creep is measured using creep tests. A force is applied and maintained for a period of time. The force is removed to reveal the reversible and irreversible deformation.
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Posted September 01, 2017.
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An automated confocal micro-extensometer enables in vivo quantification of mechanical properties with cellular resolution
Sarah Robinson, Michal Huflejt, Pierre Barbier de Reuille, Siobhan A. Braybrook, Martine Schorderet, Didier Reinhardt, Cris Kuhlemeier
bioRxiv 183533; doi: https://doi.org/10.1101/183533
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An automated confocal micro-extensometer enables in vivo quantification of mechanical properties with cellular resolution
Sarah Robinson, Michal Huflejt, Pierre Barbier de Reuille, Siobhan A. Braybrook, Martine Schorderet, Didier Reinhardt, Cris Kuhlemeier
bioRxiv 183533; doi: https://doi.org/10.1101/183533

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