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Tissue specific auxin biosynthesis regulates leaf vein patterning

Irina Kneuper, William Teale, Jonathan Edward Dawson, Ryuji Tsugeki, View ORCID ProfileKlaus Palme, View ORCID ProfileEleni Katifori, View ORCID ProfileFranck Anicet Ditengou
doi: https://doi.org/10.1101/184275
Irina Kneuper
1Institute of Biology II, Faculty of Biology, Albert-Ludwigs-University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.
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William Teale
1Institute of Biology II, Faculty of Biology, Albert-Ludwigs-University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.
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Jonathan Edward Dawson
2Physics of Biological Organization, Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077 Göttingen, Germany.
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Ryuji Tsugeki
3Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502 Japan.
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Klaus Palme
1Institute of Biology II, Faculty of Biology, Albert-Ludwigs-University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.
4Center for Biological Systems Analysis, Albert-Ludwigs-University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany.
5Freiburg Institute for Advanced Sciences (FRIAS), Albert-Ludwigs-University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany.
6BIOSS Center for Biological Signalling Studies, Albert-Ludwigs-University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany.
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Eleni Katifori
2Physics of Biological Organization, Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077 Göttingen, Germany.
7Department of Physics and Astronomy, University of Pennsylvania, PA, 19104, USA.
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Franck Anicet Ditengou
1Institute of Biology II, Faculty of Biology, Albert-Ludwigs-University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.
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  • ORCID record for Franck Anicet Ditengou
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Summary

The plant hormone auxin (indole-3-acetic acid, IAA) has a profound influence over plant cell growth and differentiation. Current understanding of vein development in leaves is based on the canalization of auxin into self-reinforcing streams which determine the sites of vascular cell differentiation. However, the role of auxin biosynthesis during leaf development in the context of leaf vein patterning has not been much studied so far. Here we characterize the context specific importance of auxin biosynthesis, auxin transport and mechanical regulations in a growing leaf. We show that domains of auxin biosynthesis predict the positioning of vascular cells. In mutants that have reduced capacity in auxin biosynthesis, leaf vein formation is decreased. While exogenous application of auxin does not compensate the loss of vein formation in auxin biosynthesis mutants, inhibition of polar auxin transport does compensate the vein-less phenotype, suggesting that the site-specific accumulation of auxin, which is likely to be mainly caused by the local auxin biosynthesis, is important for leaf vein formation. Our computational model of midvein development brings forth the interplay of cell stiffness and auxin dependent cell division. We propose that local auxin biosynthesis has the integral role in leaf vascular development.

Highlights

  • Built spatially and temporally resolved auxin biosynthesis map in growing leaf primordium of Arabidopsis.

  • Expression domains of auxin biosynthetic enzymes within primordia strongly correlated with leaf vein initiation.

  • Results show that domains of auxin biosynthesis within primordia drive leaf vein initiation and patterning.

Highlights and eTOC Blurb Using modelling and a spatiotemporal analysis of auxin biosynthesis and transport, Kneuper et al. show that tissue specific auxin biosynthesis defines places of vein initiation hence underlining the importance of auxin concentration in vein initiation.

Footnotes

  • Lead Contact Franck A. Ditengou (franck.ditengou{at}biologie.uni-freiburg.de)

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted September 04, 2017.
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Tissue specific auxin biosynthesis regulates leaf vein patterning
Irina Kneuper, William Teale, Jonathan Edward Dawson, Ryuji Tsugeki, Klaus Palme, Eleni Katifori, Franck Anicet Ditengou
bioRxiv 184275; doi: https://doi.org/10.1101/184275
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Tissue specific auxin biosynthesis regulates leaf vein patterning
Irina Kneuper, William Teale, Jonathan Edward Dawson, Ryuji Tsugeki, Klaus Palme, Eleni Katifori, Franck Anicet Ditengou
bioRxiv 184275; doi: https://doi.org/10.1101/184275

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