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A comprehensive profile of circulating RNAs in human serum

Sinan Uğur Umu, Hilde Langseth, Cecilie Bucher-Jonannessen, Bastian Fromm, Andreas Keller, Eckart Meese, Marianne Lauritzen, Magnus Leithaug, Robert Lyle, Trine Rounge
doi: https://doi.org/10.1101/186320
Sinan Uğur Umu
1Department of Research, Cancer Registry of Norway, Oslo, Norway
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Hilde Langseth
1Department of Research, Cancer Registry of Norway, Oslo, Norway
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Cecilie Bucher-Jonannessen
1Department of Research, Cancer Registry of Norway, Oslo, Norway
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Bastian Fromm
2Department of Tumor Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Nydalen, Oslo, Norway
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Andreas Keller
3Department of Clinical Bioinformatics, Saarland University, 66041, Saarbruecken, Germany
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Eckart Meese
4Department of Human Genetics, Saarland University, 66421, Homburg/Saar, Germany
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Marianne Lauritzen
1Department of Research, Cancer Registry of Norway, Oslo, Norway
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Magnus Leithaug
5Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway
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Robert Lyle
5Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway
6PharmaTox Strategic Research Initiative, School of Pharmacy, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway
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Trine Rounge
1Department of Research, Cancer Registry of Norway, Oslo, Norway
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ABSTRACT

Non-coding RNA (ncRNA) molecules have fundamental roles in cells and many are also stable in body fluids as extracellular RNAs. In this study, we used RNA sequencing (RNA-seq) to investigate the profile of small non-coding RNA (sncRNA) in human serum. We analyzed 10 billion lllumina reads from 477 serum samples, included in the Norwegian population-based Janus Serum Bank (JSB). We found that the core serum RNA repertoire includes 258 micro RNAs (miRNA), 441 piwi-interacting RNAs (piRNA), 411 transfer RNAs (tRNA), 24 small nucleolar RNAs (snoRNA), 125 small nuclear RNAs (snRNA) and 123 miscellaneous RNAs (misc-RNA). We also investigated biological and technical variation in expression, and the results suggest that many RNA molecules identified in serum contain signs of biological variation. They are therefore unlikely to be random degradation by-products. In addition, the presence of specific fragments of tRNA, snoRNA, Vault RNA and Y_RNA indicates protection from degradation. Our results suggest that many circulating RNAs in serum can be potential biomarkers.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted September 08, 2017.
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A comprehensive profile of circulating RNAs in human serum
Sinan Uğur Umu, Hilde Langseth, Cecilie Bucher-Jonannessen, Bastian Fromm, Andreas Keller, Eckart Meese, Marianne Lauritzen, Magnus Leithaug, Robert Lyle, Trine Rounge
bioRxiv 186320; doi: https://doi.org/10.1101/186320
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A comprehensive profile of circulating RNAs in human serum
Sinan Uğur Umu, Hilde Langseth, Cecilie Bucher-Jonannessen, Bastian Fromm, Andreas Keller, Eckart Meese, Marianne Lauritzen, Magnus Leithaug, Robert Lyle, Trine Rounge
bioRxiv 186320; doi: https://doi.org/10.1101/186320

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