Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Correlative live and super-resolution imaging reveals the dynamic structure of replication domains

Wanqing Xiang, M. Julia Roberti, Jean-Karim Hériché, Sebastian Huet, Stephanie Alexander, View ORCID ProfileJan Ellenberg
doi: https://doi.org/10.1101/189373
Wanqing Xiang
1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. Julia Roberti
1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jean-Karim Hériché
1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sebastian Huet
2Université de Rennes 1, Structure fédérative de recherche Biosit, 35043 Rennes, France.
3CNRS, UMR 6290, Institut Génétique et Développement de Rennes, 35043 Rennes, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephanie Alexander
1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jan Ellenberg
1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jan Ellenberg
  • For correspondence: jan.ellenberg@embl.de
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Chromosome organization in higher eukaryotes controls gene expression, DNA replication, and DNA repair. Genome mapping has revealed the functional units of chromatin at the sub-megabase scale as self-interacting regions called topologically associating domains (TADs) and showed they correspond to replication domains (RDs). A quantitative structural and dynamic description of RD behavior in the nucleus is however missing, as visualization of dynamic subdiffraction-sized RDs remains challenging. Using fluorescence labeling of RDs combined with correlative live and super-resolution microscopy in situ, we determined biophysical parameters to characterize the internal organization, spacing and mechanical coupling of RDs. We found that RDs are typically 150 nm in size and contain four co-replicating regions spaced 60 nm apart. Spatially neighboring RDs are spaced 300 nm apart and connected by highly flexible linker regions that couple their motion only below 550 nm. Our pipeline allows a robust quantitative characterization of chromosome structure in situ, and provides important biophysical parameters to understand general principles of chromatin organization.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted January 24, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Correlative live and super-resolution imaging reveals the dynamic structure of replication domains
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Correlative live and super-resolution imaging reveals the dynamic structure of replication domains
Wanqing Xiang, M. Julia Roberti, Jean-Karim Hériché, Sebastian Huet, Stephanie Alexander, Jan Ellenberg
bioRxiv 189373; doi: https://doi.org/10.1101/189373
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Correlative live and super-resolution imaging reveals the dynamic structure of replication domains
Wanqing Xiang, M. Julia Roberti, Jean-Karim Hériché, Sebastian Huet, Stephanie Alexander, Jan Ellenberg
bioRxiv 189373; doi: https://doi.org/10.1101/189373

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4384)
  • Biochemistry (9602)
  • Bioengineering (7100)
  • Bioinformatics (24889)
  • Biophysics (12626)
  • Cancer Biology (9968)
  • Cell Biology (14365)
  • Clinical Trials (138)
  • Developmental Biology (7966)
  • Ecology (12116)
  • Epidemiology (2067)
  • Evolutionary Biology (15997)
  • Genetics (10932)
  • Genomics (14746)
  • Immunology (9875)
  • Microbiology (23684)
  • Molecular Biology (9486)
  • Neuroscience (50911)
  • Paleontology (370)
  • Pathology (1540)
  • Pharmacology and Toxicology (2684)
  • Physiology (4022)
  • Plant Biology (8669)
  • Scientific Communication and Education (1510)
  • Synthetic Biology (2397)
  • Systems Biology (6442)
  • Zoology (1346)