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A novel strategy to express different antigens from one modified vaccinia Ankara vaccine vector

View ORCID ProfileK. B. Lauer, E. A. McKenzie, Y. Hall, C.P.C. Gowda, P. Klapper, R. Borrow, P.J. Vallely, M. W. Carroll, T. J. Blanchard
doi: https://doi.org/10.1101/191924
K. B. Lauer
1University of Manchester, Manchester, UK
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  • ORCID record for K. B. Lauer
E. A. McKenzie
1University of Manchester, Manchester, UK
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Y. Hall
2Public Health England, Porton Down, Salisbury, UK
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C.P.C. Gowda
1University of Manchester, Manchester, UK
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P. Klapper
1University of Manchester, Manchester, UK
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R. Borrow
1University of Manchester, Manchester, UK
3Public Health England, Manchester, UK
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P.J. Vallely
1University of Manchester, Manchester, UK
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M. W. Carroll
2Public Health England, Porton Down, Salisbury, UK
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T. J. Blanchard
1University of Manchester, Manchester, UK
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Abstract

To enhance global control of encephalitis and hepatitis caused by rabies-(RABV), Japanese encephalitis-(JEV), hepatitis B-virus (HBV), and enterovirus 71 (EV71) novel immunisation strategies are needed. Therefore, a multipathogen modified Vaccinia Ankara vector, expressing antigens from the above pathogens, was constructed. Two recombinants, one carrying the EV71 and JEV pathogen sequence and one the RABV-HBV pathogen sequence were generated. To ensure similar expression of the antigens, a T7-promoter was linked to the expression cassettes of all pathogen sequences. Direct regulation of this promoter was achieved through co-infection with a second T7-polymerase expressing MVA. Protein expression using this co-infection model of expression was demonstrated in vitro. To investigate the co-infection model of antigen delivery in vivo, a murine immunogenicity study was performed using the MVA-RABV-HBV recombinant. Although, serum antibodies against MVA were induced in all mice, no serum antibodies against RABV or HBV could be detected.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 21, 2017.
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A novel strategy to express different antigens from one modified vaccinia Ankara vaccine vector
K. B. Lauer, E. A. McKenzie, Y. Hall, C.P.C. Gowda, P. Klapper, R. Borrow, P.J. Vallely, M. W. Carroll, T. J. Blanchard
bioRxiv 191924; doi: https://doi.org/10.1101/191924
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A novel strategy to express different antigens from one modified vaccinia Ankara vaccine vector
K. B. Lauer, E. A. McKenzie, Y. Hall, C.P.C. Gowda, P. Klapper, R. Borrow, P.J. Vallely, M. W. Carroll, T. J. Blanchard
bioRxiv 191924; doi: https://doi.org/10.1101/191924

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