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Fundamentals of rapid injection molding for microfluidic cell-based assays

View ORCID ProfileUlri N. Lee, Xiaojing Su, David J. Guckenberger, Ashley M. Dostie, Tianzi Zhang, Erwin Berthier, Ashleigh B. Theberge
doi: https://doi.org/10.1101/194605
Ulri N. Lee
aDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, United States
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Xiaojing Su
aDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, United States
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David J. Guckenberger
bSalus Discovery, LLC, 110 East Main Street, Madison, WI 53703, United States
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Ashley M. Dostie
aDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, United States
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Tianzi Zhang
aDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, United States
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Erwin Berthier
aDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, United States
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Ashleigh B. Theberge
aDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, United States
cDepartment of Urology, University of Washington School of Medicine, Seattle, WA 98195, United States
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  • For correspondence: abt1@uw.edu
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Abstract

Microscale cell-based assays have demonstrated unique capabilities in reproducing important cellular behaviors for diagnostics and basic biological research. As these assays move beyond the prototyping stage and into biological and clinical research environments, there is a need to produce microscale culture platforms more rapidly, cost-effectively, and reproducibly. ‘Rapid’ injection molding is poised to meet this need as it enables some of the benefits of traditional high volume injection molding at a fraction of the cost. However, rapid injection molding has limitations due to the material and methods used for mold fabrication. Here, we characterize advantages and limitations of rapid injection molding for microfluidic device fabrication through measurement of key features for cell culture applications including channel geometry, feature consistency, floor thickness, and surface polishing. We demonstrate phase contrast and fluorescence imaging of cells grown in rapid injection molded devices and provide design recommendations to successfully utilize rapid injection molding methods for microscale cell-based assay development in academic laboratory settings.

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Posted September 28, 2017.
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Fundamentals of rapid injection molding for microfluidic cell-based assays
Ulri N. Lee, Xiaojing Su, David J. Guckenberger, Ashley M. Dostie, Tianzi Zhang, Erwin Berthier, Ashleigh B. Theberge
bioRxiv 194605; doi: https://doi.org/10.1101/194605
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Fundamentals of rapid injection molding for microfluidic cell-based assays
Ulri N. Lee, Xiaojing Su, David J. Guckenberger, Ashley M. Dostie, Tianzi Zhang, Erwin Berthier, Ashleigh B. Theberge
bioRxiv 194605; doi: https://doi.org/10.1101/194605

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