Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Nucleation of Multiple Buckled Structures in Intertwined DNA Double Helices

Sumitabha Brahmachari, Kathryn H. Gunn, Rebecca D. Giuntoli, Alfonso Mondragón, John F. Marko
doi: https://doi.org/10.1101/196345
Sumitabha Brahmachari
Department of Physics and Astronomy, and Department of Molecular Biosciences,Northwestern University, Evanston, Illinois 60208, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kathryn H. Gunn
Department of Physics and Astronomy, and Department of Molecular Biosciences,Northwestern University, Evanston, Illinois 60208, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rebecca D. Giuntoli
Department of Physics and Astronomy, and Department of Molecular Biosciences,Northwestern University, Evanston, Illinois 60208, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alfonso Mondragón
Department of Physics and Astronomy, and Department of Molecular Biosciences,Northwestern University, Evanston, Illinois 60208, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John F. Marko
Department of Physics and Astronomy, and Department of Molecular Biosciences,Northwestern University, Evanston, Illinois 60208, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

We study the statistical-mechanical properties of intertwined double-helical DNAs (DNA braids). In magnetic tweezers experiments we find that torsionally-stressed stretched braids supercoil via an abrupt buckling transition, which is associated with nucleation of a braid end loop, and that the buckled braid is characterized by proliferation of multiple domains. Differences between the mechanics of DNA braids and supercoiled single DNAs can be understood as an effect of increased bulkiness in the structure of the former. The experimental results are in accord with the predictions of a previously-described statistical-mechanical model.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
Back to top
PreviousNext
Posted September 30, 2017.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Nucleation of Multiple Buckled Structures in Intertwined DNA Double Helices
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Nucleation of Multiple Buckled Structures in Intertwined DNA Double Helices
Sumitabha Brahmachari, Kathryn H. Gunn, Rebecca D. Giuntoli, Alfonso Mondragón, John F. Marko
bioRxiv 196345; doi: https://doi.org/10.1101/196345
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Nucleation of Multiple Buckled Structures in Intertwined DNA Double Helices
Sumitabha Brahmachari, Kathryn H. Gunn, Rebecca D. Giuntoli, Alfonso Mondragón, John F. Marko
bioRxiv 196345; doi: https://doi.org/10.1101/196345

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (2516)
  • Biochemistry (4964)
  • Bioengineering (3466)
  • Bioinformatics (15166)
  • Biophysics (6885)
  • Cancer Biology (5379)
  • Cell Biology (7709)
  • Clinical Trials (138)
  • Developmental Biology (4518)
  • Ecology (7128)
  • Epidemiology (2059)
  • Evolutionary Biology (10206)
  • Genetics (7497)
  • Genomics (9763)
  • Immunology (4821)
  • Microbiology (13174)
  • Molecular Biology (5128)
  • Neuroscience (29354)
  • Paleontology (203)
  • Pathology (835)
  • Pharmacology and Toxicology (1460)
  • Physiology (2127)
  • Plant Biology (4728)
  • Scientific Communication and Education (1008)
  • Synthetic Biology (1337)
  • Systems Biology (4001)
  • Zoology (768)