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Impaired phloem loading in genome-edited triple knock-out mutants of SWEET13 sucrose transporters

Margaret Bezrutczyk, Thomas Hartwig, Marc Horshman, Si Nian Char, Jinliang Yang, Bing Yang, Wolf B. Frommer, Davide Sosso
doi: https://doi.org/10.1101/197921
Margaret Bezrutczyk
1Institute for Molecular Physiology, Heinrich Heine University Düsseldorf, and Max Planck Institute for Plant Breeding Research, Cologne, Germany
2Department of Plant Biology, Carnegie Science, 260 Panama St., Stanford, CA 94305, USA
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Thomas Hartwig
1Institute for Molecular Physiology, Heinrich Heine University Düsseldorf, and Max Planck Institute for Plant Breeding Research, Cologne, Germany
2Department of Plant Biology, Carnegie Science, 260 Panama St., Stanford, CA 94305, USA
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Marc Horshman
2Department of Plant Biology, Carnegie Science, 260 Panama St., Stanford, CA 94305, USA
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Si Nian Char
3Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA
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Jinliang Yang
4Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
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Bing Yang
3Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA
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Wolf B. Frommer
1Institute for Molecular Physiology, Heinrich Heine University Düsseldorf, and Max Planck Institute for Plant Breeding Research, Cologne, Germany
2Department of Plant Biology, Carnegie Science, 260 Panama St., Stanford, CA 94305, USA
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  • For correspondence: frommew@hhu.de
Davide Sosso
2Department of Plant Biology, Carnegie Science, 260 Panama St., Stanford, CA 94305, USA
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Summary

Crop yield depends on efficient allocation of sucrose from leaves to seeds. In Arabidopsis, phloem loading is mediated by a combination of SWEET sucrose effluxers and subsequent uptake by SUT1/SUC2 sucrose/H+ symporters. ZmSUT1 is essential for carbon allocation in maize, but the relative contribution to apoplasmic phloem loading and retrieval of sucrose leaking from the translocation path is not known. We therefor tested whether SWEETs are important for phloem loading in maize.

Here we identified three leaf-expressed SWEET sucrose transporters as key components of apoplasmic phloem loading in Zea mays L. Notably, ZmSWEET13 paralogs (a, b, c) are among the highest expressed genes in the leaf vasculature. Genome-edited triple knock-out mutants are severely stunted. Photosynthesis of mutants was impaired and leaves accumulated starch and soluble sugars. RNA-seq revealed profound transcriptional deregulation of genes associated with the photosynthetic apparatus and carbohydrate metabolism. GWAS analyses may indicate that variability in ZmSWEET13s is correlated with agronomical traits, specifically flowering time and leaf angle.

This work provides support for cooperation of three ZmSWEET13s with ZmSUT1 in phloem loading in Zea mays L. Our study highlights these three ZmSWEET13 sucrose transporters as possible candidates for the engineering of crop yield.

One Sentence Summary: Three SWEET sucrose transporter paralogs are necessary for phloem loading in maize.

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Posted October 06, 2017.
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Impaired phloem loading in genome-edited triple knock-out mutants of SWEET13 sucrose transporters
Margaret Bezrutczyk, Thomas Hartwig, Marc Horshman, Si Nian Char, Jinliang Yang, Bing Yang, Wolf B. Frommer, Davide Sosso
bioRxiv 197921; doi: https://doi.org/10.1101/197921
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Impaired phloem loading in genome-edited triple knock-out mutants of SWEET13 sucrose transporters
Margaret Bezrutczyk, Thomas Hartwig, Marc Horshman, Si Nian Char, Jinliang Yang, Bing Yang, Wolf B. Frommer, Davide Sosso
bioRxiv 197921; doi: https://doi.org/10.1101/197921

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