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The cell cycle gene regulatory DREAM complex is disrupted by high expression of oncogenic B-Myb

Audra N. Iness, Jessica Felthousen, Varsha Ananthapadmanabhan, Keelan Z. Guiley, Mikhail Dozmorov, Seth M. Rubin, Larisa Litovchick
doi: https://doi.org/10.1101/199539
Audra N. Iness
1Division of Hematology, Oncology and Palliative Care and Massey Cancer Center, Virginia Commonwealth University, Richmond, VA 23298, USA
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Jessica Felthousen
1Division of Hematology, Oncology and Palliative Care and Massey Cancer Center, Virginia Commonwealth University, Richmond, VA 23298, USA
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Varsha Ananthapadmanabhan
1Division of Hematology, Oncology and Palliative Care and Massey Cancer Center, Virginia Commonwealth University, Richmond, VA 23298, USA
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Keelan Z. Guiley
2Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
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Mikhail Dozmorov
3Department of Biostatistics, Virginia Commonwealth University, Richmond, Virginia, 23298, USA
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Seth M. Rubin
2Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
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Larisa Litovchick
1Division of Hematology, Oncology and Palliative Care and Massey Cancer Center, Virginia Commonwealth University, Richmond, VA 23298, USA
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  • For correspondence: larisa.litovchick@vcuhealth.org
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Abstract

The oncogene MYBL2 (encoding B-Myb) is a poor prognostic biomarker in many cancers. B-Myb interacts with the MuvB core of five proteins (LIN9, LIN37, LIN52, LIN53/RBBP4, and LIN54) to form the MMB (Myb-MuvB) complex and promotes expression of late cell cycle genes necessary for progression through mitosis. Both MYBL2 amplification and over-expression are associated with deregulation of the cell cycle and increased cell proliferation. Alternatively, by interacting with E2F4-DP1 and p130 or p107, the MuvB core becomes part of the DREAM complex (DP, RB-like, E2F, and MuvB). The DREAM complex opposes MMB by globally repressing cell cycle genes in G0/G1, maintaining the cell in a quiescent state. However, the specific mechanism by which B-Myb alters the cell cycle is not well understood. Our analysis of The Cancer Genome Atlas data revealed significant upregulation of DREAM and MMB target genes in breast and ovarian cancer with MYBL2 gain. Given that most of the DREAM target genes are not directly regulated by B-Myb, we investigated the effects of B-Myb on DREAM formation. We found that depletion of B-Myb results in increased DREAM formation in human cancer cells, while its overexpression inhibits DREAM formation in the non-transformed cells. Since the MuvB core subunit LIN52 is essential for assembly of both the DREAM and MMB complexes, we tested whether B-Myb disrupts DREAM by sequestering LIN52. Overexpression of LIN52 did not increase either DREAM or MMB formation, but instead increased the turnover rate of the endogenous LIN52 protein. Interestingly, co-expression of B-Myb increased the expression of both endogenous and overexpressed LIN52 while knockdown of B-Myb had an opposite effect. We found that regulation of LIN52 occurs at the protein level, and that activity of DYRK1A kinase, the enzyme that triggers DREAM complex formation by phosphorylating LIN52, is required for this regulation. These findings are the first to implicate B-Myb in the disassembly of the DREAM complex and offer insight into the underlying mechanisms of poor prognostic value of MYBL2 amplification in cancer. We conclude that B-Myb mediates its oncogenic effects not only by increasing mitotic gene expression by the MMB complex, but also by broad disruption of cell cycle gene regulatory programs through compromised DREAM formation.

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Posted October 06, 2017.
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The cell cycle gene regulatory DREAM complex is disrupted by high expression of oncogenic B-Myb
Audra N. Iness, Jessica Felthousen, Varsha Ananthapadmanabhan, Keelan Z. Guiley, Mikhail Dozmorov, Seth M. Rubin, Larisa Litovchick
bioRxiv 199539; doi: https://doi.org/10.1101/199539
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The cell cycle gene regulatory DREAM complex is disrupted by high expression of oncogenic B-Myb
Audra N. Iness, Jessica Felthousen, Varsha Ananthapadmanabhan, Keelan Z. Guiley, Mikhail Dozmorov, Seth M. Rubin, Larisa Litovchick
bioRxiv 199539; doi: https://doi.org/10.1101/199539

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