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Population-scale organization of cerebellar granule neuron signaling during a visuomotor behavior

Sherika J.G. Sylvester, Melanie M. Lee, Alexandro Ramirez, Sukbin Lim, Mark S. Goldman, Emre R.F. Aksay
doi: https://doi.org/10.1101/200824
Sherika J.G. Sylvester
1Institute for Computational Biomedicine and the Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, USA, 10021.
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Melanie M. Lee
1Institute for Computational Biomedicine and the Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, USA, 10021.
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Alexandro Ramirez
1Institute for Computational Biomedicine and the Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, USA, 10021.
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Sukbin Lim
2Center for Neuroscience, University of California at Davis, Davis, California, USA
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Mark S. Goldman
2Center for Neuroscience, University of California at Davis, Davis, California, USA
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Emre R.F. Aksay
1Institute for Computational Biomedicine and the Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, USA, 10021.
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ABSTRACT

Granule cells at the input layer of the cerebellum comprise over half the neurons in the human brain and are thought to be critical for learning. However, little is known about granule neuron signaling at the population scale during behavior. We used calcium imaging in awake zebrafish during optokinetic behavior to record transgenically identified granule neurons throughout a cerebellar population. A significant fraction of the population was responsive at any given time. In contrast to core precerebellar populations, granule neuron responses were relatively heterogeneous, with variation in the degree of rectification and the balance of excitation versus inhibition. Functional correlations were strongest for nearby cells, with weak spatial gradients in the degree of rectification and excitation. These data open a new window upon cerebellar function and suggest granule layer signals represent elementary building blocks underrepresented in core sensorimotor pathways, thereby enabling the construction of novel patterns of activity for learning.

SIGNIFICANCE STATEMENT Cerebellar processing is important for a variety of fine motor tasks and sensorimotor adaptations, and a growing body of evidence indicates a prominent role in cognitive control. However, it has been challenging to understand cerebellar function during behavior because of difficulties in recording from cerebellar granule neurons, the most populous neuron type in the brain. We use population-scale optical imaging in the larval zebrafish to compare precerebellar activity to granule cell signaling. Our results suggest a behaviorally relevant expansion of precerebellar signaling representations at the granule layer of the cerebellum.

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  • Conflict of Interest: None

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 10, 2017.
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Population-scale organization of cerebellar granule neuron signaling during a visuomotor behavior
Sherika J.G. Sylvester, Melanie M. Lee, Alexandro Ramirez, Sukbin Lim, Mark S. Goldman, Emre R.F. Aksay
bioRxiv 200824; doi: https://doi.org/10.1101/200824
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Population-scale organization of cerebellar granule neuron signaling during a visuomotor behavior
Sherika J.G. Sylvester, Melanie M. Lee, Alexandro Ramirez, Sukbin Lim, Mark S. Goldman, Emre R.F. Aksay
bioRxiv 200824; doi: https://doi.org/10.1101/200824

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