Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Unconventional kinetochore kinases KKT2 and KKT3 have a unique zinc finger that promotes their kinetochore localization

View ORCID ProfileGabriele Marcianò, Olga O. Nerusheva, View ORCID ProfileMidori Ishii, View ORCID ProfileBungo Akiyoshi
doi: https://doi.org/10.1101/2019.12.13.875419
Gabriele Marcianò
Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Gabriele Marcianò
Olga O. Nerusheva
Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Midori Ishii
Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Midori Ishii
Bungo Akiyoshi
Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Bungo Akiyoshi
  • For correspondence: bungo.akiyoshi@bioch.ox.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Chromosome segregation in eukaryotes is driven by the kinetochore, a macromolecular protein complex that assembles onto centromeric DNA and binds spindle microtubules. Cells must tightly control the number and position of kinetochores so that all chromosomes assemble a single kinetochore. A central player in this process is the centromere-specific histone H3 variant CENP-A, which localizes specifically within centromeres and promotes kinetochore assembly. However, CENP-A is absent from several eukaryotic lineages including kinetoplastids, a group of evolutionarily divergent eukaryotes that have an unconventional set of kinetochore proteins. It remains unknown how kinetoplastids specify kinetochore positions or promote kinetochore assembly in the absence of CENP-A. Here we studied two homologous kinetoplastid kinases (KKT2 and KKT3) that localize constitutively at centromeres. KKT2 and KKT3 central domains were sufficient for centromere localization in Trypanosoma brucei. Crystal structures of the KKT2 central domain from two divergent kinetoplastids revealed a unique zinc finger domain, which promotes its kinetochore localization in T. brucei. Mutations in the equivalent zinc finger domain of KKT3 abolished its kinetochore localization and function. This study lays the foundation for understanding the mechanism of kinetochore specification and assembly in kinetoplastids.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted December 13, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Unconventional kinetochore kinases KKT2 and KKT3 have a unique zinc finger that promotes their kinetochore localization
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Unconventional kinetochore kinases KKT2 and KKT3 have a unique zinc finger that promotes their kinetochore localization
Gabriele Marcianò, Olga O. Nerusheva, Midori Ishii, Bungo Akiyoshi
bioRxiv 2019.12.13.875419; doi: https://doi.org/10.1101/2019.12.13.875419
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Unconventional kinetochore kinases KKT2 and KKT3 have a unique zinc finger that promotes their kinetochore localization
Gabriele Marcianò, Olga O. Nerusheva, Midori Ishii, Bungo Akiyoshi
bioRxiv 2019.12.13.875419; doi: https://doi.org/10.1101/2019.12.13.875419

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4085)
  • Biochemistry (8755)
  • Bioengineering (6477)
  • Bioinformatics (23331)
  • Biophysics (11740)
  • Cancer Biology (9144)
  • Cell Biology (13237)
  • Clinical Trials (138)
  • Developmental Biology (7410)
  • Ecology (11364)
  • Epidemiology (2066)
  • Evolutionary Biology (15084)
  • Genetics (10397)
  • Genomics (14006)
  • Immunology (9115)
  • Microbiology (22036)
  • Molecular Biology (8777)
  • Neuroscience (47345)
  • Paleontology (350)
  • Pathology (1420)
  • Pharmacology and Toxicology (2480)
  • Physiology (3703)
  • Plant Biology (8045)
  • Scientific Communication and Education (1431)
  • Synthetic Biology (2207)
  • Systems Biology (6014)
  • Zoology (1249)