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MSL3 coordinates a transcriptional and translational meiotic program in female Drosophila

Alicia McCarthy, Kahini Sarkar, Elliot T Martin, Maitreyi Upadhyay, Joshua R James, Jennifer M Lin, Seoyeon Jang, Nathan D Williams, Paolo E Forni, Michael Buszczak, Prashanth Rangan
doi: https://doi.org/10.1101/2019.12.18.879874
Alicia McCarthy
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
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Kahini Sarkar
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
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Elliot T Martin
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
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Maitreyi Upadhyay
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
2#Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138
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Joshua R James
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
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Jennifer M Lin
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
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Seoyeon Jang
3Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390
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Nathan D Williams
3Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390
4#Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
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Paolo E Forni
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
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Michael Buszczak
3Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390
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Prashanth Rangan
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
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  • For correspondence: prangan@albany.edu
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Summary

Gamete formation from germline stem cells (GSCs) is essential for sexual reproduction. However, the regulation of GSC differentiation and meiotic entry are incompletely understood. Set2, which deposits H3K36me3 modifications, is required for differentiation of GSCs during Drosophila oogenesis. We discovered that the H3K36me3 reader Male-specific lethal 3 (MSL3) and the histone acetyltransferase complex Ada2a-containing (ATAC) cooperate with Set2 to regulate entry into meiosis in female Drosophila. MSL3 expression is restricted to the mitotic and early meiotic stages of the female germline, where it promotes transcription of genes encoding synaptonemal complex components and a germline enriched ribosomal protein S19 paralog, RpS19b. RpS19b upregulation is required for translation of Rbfox1, a known meiotic cell cycle entry factor. Thus, MSL3 is a master regulator of meiosis, coordinating the expression of factors required for recombination and GSC differentiation. We find that MSL3 is expressed during mouse spermatogenesis, suggesting a conserved function during meiosis.

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Posted December 19, 2019.
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MSL3 coordinates a transcriptional and translational meiotic program in female Drosophila
Alicia McCarthy, Kahini Sarkar, Elliot T Martin, Maitreyi Upadhyay, Joshua R James, Jennifer M Lin, Seoyeon Jang, Nathan D Williams, Paolo E Forni, Michael Buszczak, Prashanth Rangan
bioRxiv 2019.12.18.879874; doi: https://doi.org/10.1101/2019.12.18.879874
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MSL3 coordinates a transcriptional and translational meiotic program in female Drosophila
Alicia McCarthy, Kahini Sarkar, Elliot T Martin, Maitreyi Upadhyay, Joshua R James, Jennifer M Lin, Seoyeon Jang, Nathan D Williams, Paolo E Forni, Michael Buszczak, Prashanth Rangan
bioRxiv 2019.12.18.879874; doi: https://doi.org/10.1101/2019.12.18.879874

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