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Temporal specificity and heterogeneity of the fly immune cells’ transcriptional landscape

View ORCID ProfilePierre B. Cattenoz, Rosy Sakr, Alexia Pavlidaki, Claude Delaporte, Andrea Riba, View ORCID ProfileNacho Molina, View ORCID ProfileNivedita Hariharan, View ORCID ProfileTina Mukherjee, View ORCID ProfileAngela Giangrande
doi: https://doi.org/10.1101/2019.12.20.871301
Pierre B. Cattenoz
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France
4Université de Strasbourg, Illkirch, France
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  • ORCID record for Pierre B. Cattenoz
  • For correspondence: angela@igbmc.fr cattenoz@igbmc.fr
Rosy Sakr
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France
4Université de Strasbourg, Illkirch, France
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Alexia Pavlidaki
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France
4Université de Strasbourg, Illkirch, France
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Claude Delaporte
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France
4Université de Strasbourg, Illkirch, France
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Andrea Riba
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France
4Université de Strasbourg, Illkirch, France
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Nacho Molina
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France
4Université de Strasbourg, Illkirch, France
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  • ORCID record for Nacho Molina
Nivedita Hariharan
5Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK PO Bellary Road, Bangalore 560065, India
6The University of Trans-disciplinary Health Sciences and Technology, Bangalore 560064, India
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Tina Mukherjee
5Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK PO Bellary Road, Bangalore 560065, India
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Angela Giangrande
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France
4Université de Strasbourg, Illkirch, France
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  • ORCID record for Angela Giangrande
  • For correspondence: angela@igbmc.fr cattenoz@igbmc.fr
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Summary

Immune cells provide defense against the non-self, however recent data suggest roles well beyond innate immunity, in processes as diverse as development, metabolism and tumor progression. Nevertheless, the heterogeneity of these cells remains an open question. Using bulk RNA sequencing we find that the Drosophila immune cells (hemocytes) display distinct features in the embryo, a closed and rapidly developing system, compared to the larva, which is exposed to environmental and metabolic challenges. Through single cell RNA sequencing we identify fourteen hemocyte clusters present in unchallenged larvae and associated with distinct cellular processes e.g. proliferation, phagocytosis, metabolic homeostasis and humoral response. Finally, we characterize the changes occurring in the hemocyte clusters upon wasp infestation that triggers the differentiation of a novel cell type, the lamellocyte. This first molecular atlas provides precious insights and paves the way to study the biology of the Drosophila immune cells in physiological and pathological conditions.

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Posted December 20, 2019.
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Temporal specificity and heterogeneity of the fly immune cells’ transcriptional landscape
Pierre B. Cattenoz, Rosy Sakr, Alexia Pavlidaki, Claude Delaporte, Andrea Riba, Nacho Molina, Nivedita Hariharan, Tina Mukherjee, Angela Giangrande
bioRxiv 2019.12.20.871301; doi: https://doi.org/10.1101/2019.12.20.871301
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Temporal specificity and heterogeneity of the fly immune cells’ transcriptional landscape
Pierre B. Cattenoz, Rosy Sakr, Alexia Pavlidaki, Claude Delaporte, Andrea Riba, Nacho Molina, Nivedita Hariharan, Tina Mukherjee, Angela Giangrande
bioRxiv 2019.12.20.871301; doi: https://doi.org/10.1101/2019.12.20.871301

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