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On-demand spatiotemporal programming of collective cell migration via bioelectric stimulation

View ORCID ProfileTom J. Zajdel, Gawoon Shim, Linus Wang, Alejandro Rossello-Martinez, View ORCID ProfileDaniel J. Cohen
doi: https://doi.org/10.1101/2019.12.20.884510
Tom J. Zajdel
aDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, United States
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Gawoon Shim
aDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, United States
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Linus Wang
aDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, United States
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Alejandro Rossello-Martinez
aDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, United States
bDepartment of Aeronautics, Imperial College London, London SW7 2AZ, United Kingdom
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Daniel J. Cohen
aDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, United States
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  • For correspondence: danielcohen@princeton.edu
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Abstract

Directed cell migration is critical across biological processes spanning healing to cancer invasion, yet no tools allow such migration to be interactively guided. We present a new bioreactor that harnesses electrotaxis—directed cell migration along electric field gradients—by integrating multiple independent electrodes under computer control to dynamically program electric field patterns, and hence steer cell migration. Using this platform, we programmed and characterized multiple precise, two-dimensional collective migration maneuvers in renal epithelia and primary skin keratinocyte ensembles. First, we demonstrated on-demand, 90-degree collective turning. Next, we developed a universal electrical stimulation scheme capable of programming arbitrary 2D migration maneuvers such as precise angular turns and directing cells to migrate in a complete circle. Our stimulation scheme proves that cells effectively timeaverage electric field cues, helping to elucidate the transduction time scales in electrotaxis. Together, this work represents a fundamentally different platform for controlling cell migration with broad utility across fields.

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Posted December 23, 2019.
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On-demand spatiotemporal programming of collective cell migration via bioelectric stimulation
Tom J. Zajdel, Gawoon Shim, Linus Wang, Alejandro Rossello-Martinez, Daniel J. Cohen
bioRxiv 2019.12.20.884510; doi: https://doi.org/10.1101/2019.12.20.884510
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On-demand spatiotemporal programming of collective cell migration via bioelectric stimulation
Tom J. Zajdel, Gawoon Shim, Linus Wang, Alejandro Rossello-Martinez, Daniel J. Cohen
bioRxiv 2019.12.20.884510; doi: https://doi.org/10.1101/2019.12.20.884510

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