Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

SCHEEPDOG: programming electric cues to dynamically herd large-scale cell migration

View ORCID ProfileTom J. Zajdel, Gawoon Shim, Linus Wang, Alejandro Rossello-Martinez, View ORCID ProfileDaniel J. Cohen
doi: https://doi.org/10.1101/2019.12.20.884510
Tom J. Zajdel
aDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Tom J. Zajdel
Gawoon Shim
aDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Linus Wang
aDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alejandro Rossello-Martinez
aDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544
bDepartment of Aeronautics, Imperial College London, London SW7 2AZ, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel J. Cohen
aDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Daniel J. Cohen
  • For correspondence: danielcohen@princeton.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Directed cell migration is critical across biological processes spanning healing to cancer invasion, yet no existing tools allow real-time interactive guidance over such migration. We present a new bioreactor that harnesses electrotaxis— directed cell migration along electric field gradients—by integrating four independent electrodes under computer control to dynamically program electric field patterns, and hence steer cell migration. Using this platform, we programmed and characterized multiple precise, two-dimensional collective migration maneuvers in renal epithelia and primary skin keratinocyte ensembles. First, we demonstrated on-demand, 90-degree collective turning. Next, we developed a universal electrical stimulation scheme capable of programming arbitrary 2D migration maneuvers such as precise angular turns and migration in a complete circle. Our stimulation scheme proves that cells effectively time-average electric field cues, helping to elucidate the transduction time scales in electrotaxis. Together, this work represents an enabling platform for controlling cell migration with broad utility across many cell types.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Revised Figs. 2 & 3 and added Supplementary Figs. 3 & 4.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted April 28, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
SCHEEPDOG: programming electric cues to dynamically herd large-scale cell migration
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
SCHEEPDOG: programming electric cues to dynamically herd large-scale cell migration
Tom J. Zajdel, Gawoon Shim, Linus Wang, Alejandro Rossello-Martinez, Daniel J. Cohen
bioRxiv 2019.12.20.884510; doi: https://doi.org/10.1101/2019.12.20.884510
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
SCHEEPDOG: programming electric cues to dynamically herd large-scale cell migration
Tom J. Zajdel, Gawoon Shim, Linus Wang, Alejandro Rossello-Martinez, Daniel J. Cohen
bioRxiv 2019.12.20.884510; doi: https://doi.org/10.1101/2019.12.20.884510

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Bioengineering
Subject Areas
All Articles
  • Animal Behavior and Cognition (4397)
  • Biochemistry (9619)
  • Bioengineering (7113)
  • Bioinformatics (24917)
  • Biophysics (12643)
  • Cancer Biology (9980)
  • Cell Biology (14389)
  • Clinical Trials (138)
  • Developmental Biology (7980)
  • Ecology (12135)
  • Epidemiology (2067)
  • Evolutionary Biology (16011)
  • Genetics (10940)
  • Genomics (14766)
  • Immunology (9891)
  • Microbiology (23720)
  • Molecular Biology (9495)
  • Neuroscience (51025)
  • Paleontology (370)
  • Pathology (1544)
  • Pharmacology and Toxicology (2688)
  • Physiology (4031)
  • Plant Biology (8687)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2403)
  • Systems Biology (6449)
  • Zoology (1346)