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DRB1 as a mediator between transcription and microRNA processing

View ORCID ProfileDawid Bielewicz, Jakub Dolata, Mateusz Bajczyk, Lukasz Szewc, Tomasz Gulanicz, Susheel Sagar Bhat, Anna Karlik, Artur Jarmolowski, Zofia Szweykowska-Kulinska
doi: https://doi.org/10.1101/2019.12.30.890665
Dawid Bielewicz
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland
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  • ORCID record for Dawid Bielewicz
  • For correspondence: bieda@amu.edu.pl
Jakub Dolata
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland
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Mateusz Bajczyk
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland
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Lukasz Szewc
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland
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Tomasz Gulanicz
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland
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Susheel Sagar Bhat
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland
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Anna Karlik
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland
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Artur Jarmolowski
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland
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Zofia Szweykowska-Kulinska
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland
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Abstract

DRB1 (HYL1) is a double-stranded RNA binding protein involved in miRNA processing in plants. It is a core component of the Microprocessor complex and enhances the efficiency and precision of miRNA processing by DCL1 protein. In this work, we report a novel function of DRB1 protein in the transcription of MIR genes. DRB1 co-localizes with RNA Polymerase II and affects its distribution along MIR genes. Moreover, proteomic experiments revealed that DRB1 protein interacts with many transcription factors. Finally, we show that the action of DRB1 is not limited to MIR genes as it impacts expression of many other genes, majority of which are involved in plant response to light. These discoveries add DRB1 as another player of gene regulation at transcriptional level, independent of its role in miRNA biogenesis.

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Posted December 30, 2019.
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DRB1 as a mediator between transcription and microRNA processing
Dawid Bielewicz, Jakub Dolata, Mateusz Bajczyk, Lukasz Szewc, Tomasz Gulanicz, Susheel Sagar Bhat, Anna Karlik, Artur Jarmolowski, Zofia Szweykowska-Kulinska
bioRxiv 2019.12.30.890665; doi: https://doi.org/10.1101/2019.12.30.890665
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DRB1 as a mediator between transcription and microRNA processing
Dawid Bielewicz, Jakub Dolata, Mateusz Bajczyk, Lukasz Szewc, Tomasz Gulanicz, Susheel Sagar Bhat, Anna Karlik, Artur Jarmolowski, Zofia Szweykowska-Kulinska
bioRxiv 2019.12.30.890665; doi: https://doi.org/10.1101/2019.12.30.890665

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