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Improved subspecies identification in clinical Mycobacterium abscessus complex isolates using whole genome sequence

Dachuan Lin, Ruifeng Sun, Yaoju Tan, Jing Wang, Xinchun Chen
doi: https://doi.org/10.1101/2020.01.02.893412
Dachuan Lin
aGuangdong Key Laboratory of Regional Immunity and Diseases, Department of Pathogen Biology, Shenzhen University School of Medicine, Shenzhen 518054, China
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Ruifeng Sun
bOffice of Student Resources and Residential Life, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
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Yaoju Tan
cState Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital, Guangzhou 510095, China
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Jing Wang
dKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou 225009, China
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Xinchun Chen
aGuangdong Key Laboratory of Regional Immunity and Diseases, Department of Pathogen Biology, Shenzhen University School of Medicine, Shenzhen 518054, China
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  • For correspondence: chenxinchun@szu.edu.cn
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Abstract

Mycobacterium abscessus complex, which is frequently reported causing a variety of skin and soft tissues diseases in humans, is composed of three subspecies, namely M. abscessus subsp. abscessus, M. abscessus subsp. massiliense and M. abscessus subsp. bolletii. Currently, the differentiation of these three subspecies in clinical isolates still largely depend on single gene identification methods like the genes namely hsp65, 16s with a limited accuracy. This study confirmed the limitations of the single gene based method in the subspecies identification. We performed a comprehensive analysis of MABC genomes in the NCBI database and tried to build an accurate and user-friendly identify method. Here, we describe an improved assay for Mycobacterium abscessus complex fast identification using WGS data, based on the identities of rpoB, erm(41) and rpls. Comprehensive analysis has been performed to compare our software results with the traditional method. The result showed that the method built-in this study could 100% identification the subspecies for the Mycobacterium abscessus complex in the public genome database (893 genomes from NCBI database and 6 clinical isolates from this study). Because this software can be easily integrated into a routine workflow to quickly and precisely provide subspecies-level identification and discrimination MABC different subspecies in clinical isolates by WGS. This assay will facilitate accurate molecular identification of species from the MABC complex in a variety of clinical specimens and diagnostic contexts.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 03, 2020.
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Improved subspecies identification in clinical Mycobacterium abscessus complex isolates using whole genome sequence
Dachuan Lin, Ruifeng Sun, Yaoju Tan, Jing Wang, Xinchun Chen
bioRxiv 2020.01.02.893412; doi: https://doi.org/10.1101/2020.01.02.893412
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Improved subspecies identification in clinical Mycobacterium abscessus complex isolates using whole genome sequence
Dachuan Lin, Ruifeng Sun, Yaoju Tan, Jing Wang, Xinchun Chen
bioRxiv 2020.01.02.893412; doi: https://doi.org/10.1101/2020.01.02.893412

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