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Engineering E. coli for magnetic control and the spatial localization of functions

Mary Aubry, Wei-An Wang, Yohan Guyodo, Eugénia Delacou, Jean Michel Guignier, Olivier Espeli, View ORCID ProfileAlice Lebreton, François Guyot, Zoher Gueroui
doi: https://doi.org/10.1101/2020.01.06.895623
Mary Aubry
PASTEUR, Department of Chemistry, École Normale Supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, France
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Wei-An Wang
PASTEUR, Department of Chemistry, École Normale Supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, FranceIMPMC, Muséum National d’Histoire Naturelle, Sorbonne Université, UMR CNRS 7590, 75005 Paris, France
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Yohan Guyodo
Université de Paris, Institut de physique du globe de Paris, CNRS, F-75005 Paris, France
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Eugénia Delacou
PASTEUR, Department of Chemistry, École Normale Supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, France
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Jean Michel Guignier
IMPMC, Muséum National d’Histoire Naturelle, Sorbonne Université, UMR CNRS 7590, 75005 Paris, France
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Olivier Espeli
CIRB-Collège de France, CNRS-UMR724, INSERM U1050, PSL Research University, Paris, France
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Alice Lebreton
Institut de biologie de l’ENS (IBENS), Département de biologie, École normale supérieure, CNRS, INSERM, PSL University, 75005 Paris, FranceINRAE, IBENS, 75005 Paris, France
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  • ORCID record for Alice Lebreton
François Guyot
IMPMC, Muséum National d’Histoire Naturelle, Sorbonne Université, UMR CNRS 7590, 75005 Paris, FranceInstitut Universitaire de France (IUF)
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Zoher Gueroui
PASTEUR, Department of Chemistry, École Normale Supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, France
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  • For correspondence: zoher.gueroui@ens.fr
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Abstract

The fast-developing field of synthetic biology enables broad applications of programmed microorganisms including the development of whole-cell biosensors, delivery vehicles for therapeutics, or diagnostic agents. However, the lack of spatial control required for localizing microbial functions could limit their use and induce their dilution leading to ineffective action or dissemination. To overcome this limitation, the integration of magnetic properties into living systems enables a contact-less and orthogonal method for spatiotemporal control. Here, we generated a magnetic-sensing Escherichia coli by driving the formation of iron-rich bodies into bacteria. We found that these bacteria could be spatially controlled by magnetic forces and sustained cell growth and division, by transmitting asymmetrically their magnetic properties to one daughter cell. We combined the spatial control of bacteria with genetically encoded-adhesion properties to achieve the magnetic capture of specific target bacteria as well as the spatial modulation of human cell invasions.

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Posted January 07, 2020.
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Engineering E. coli for magnetic control and the spatial localization of functions
Mary Aubry, Wei-An Wang, Yohan Guyodo, Eugénia Delacou, Jean Michel Guignier, Olivier Espeli, Alice Lebreton, François Guyot, Zoher Gueroui
bioRxiv 2020.01.06.895623; doi: https://doi.org/10.1101/2020.01.06.895623
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Engineering E. coli for magnetic control and the spatial localization of functions
Mary Aubry, Wei-An Wang, Yohan Guyodo, Eugénia Delacou, Jean Michel Guignier, Olivier Espeli, Alice Lebreton, François Guyot, Zoher Gueroui
bioRxiv 2020.01.06.895623; doi: https://doi.org/10.1101/2020.01.06.895623

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