Abstract
The laboratory reference strain 630Δerm of the anaerobic human pathogen Clostridioides difficile is characterized by a remarkable high oxygen tolerance. We show that an amino acid exchange in the DNA binding domain of the hydrogen peroxide sensor PerR results in a constitutive derepression of PerR-controlled genes and thus in an oxidative stress response even under anaerobic conditions. This questions the model status, strain 630Δerm claims in C. difficile research.
Footnotes
Competing Interest Statement: The authors declare no competing interests.
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