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In vivo control of the ezrin/radixin/moesin protein ERM-1 in C. elegans

View ORCID ProfileJoão J. Ramalho, Ophélie Nicolle, View ORCID ProfileGrégoire Michaux, View ORCID ProfileMike Boxem
doi: https://doi.org/10.1101/2020.01.08.898189
João J. Ramalho
1Division of Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
3Present address: Laboratory of Biochemistry, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands
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Ophélie Nicolle
2Univ Rennes, CNRS, IGDR (Institut de Génétique et de Développement de Rennes), UMR 6290, F-35000 Rennes, France
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Grégoire Michaux
2Univ Rennes, CNRS, IGDR (Institut de Génétique et de Développement de Rennes), UMR 6290, F-35000 Rennes, France
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Mike Boxem
1Division of Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
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  • For correspondence: m.boxem@uu.nl
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Abstract

ERM proteins are conserved regulators of cortical membrane specialization, that function as membrane–actin linkers and molecular hubs. Activity of ERM proteins requires a conformational switch from an inactive cytoplasmic form into an active membrane- and actin-bound form, which is thought to be mediated by sequential PIP2-binding and phosphorylation of a conserved C-terminal threonine residue. Here, we use the single C. elegans ERM ortholog, ERM-1, to study the contribution of these regulatory events to ERM activity and tissue formation in vivo. Using CRISPR/Cas9-generated erm-1 mutant alleles we demonstrate that PIP2-binding is critically required for ERM-1 function. In contrast, dynamic regulation of C-terminal T544 phosphorylation is not essential but modulates ERM-1 apical localization and dynamics in a tissue-specific manner, to control cortical actin organization and drive lumen formation in epithelial tubes. Our work highlights the dynamic nature of ERM protein regulation during tissue morphogenesis and the importance of C-terminal phosphorylation in fine-tuning ERM activity in a tissue-specific context.

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Posted January 08, 2020.
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In vivo control of the ezrin/radixin/moesin protein ERM-1 in C. elegans
João J. Ramalho, Ophélie Nicolle, Grégoire Michaux, Mike Boxem
bioRxiv 2020.01.08.898189; doi: https://doi.org/10.1101/2020.01.08.898189
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In vivo control of the ezrin/radixin/moesin protein ERM-1 in C. elegans
João J. Ramalho, Ophélie Nicolle, Grégoire Michaux, Mike Boxem
bioRxiv 2020.01.08.898189; doi: https://doi.org/10.1101/2020.01.08.898189

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