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Bright and tunable far-red chemigenetic indicators

Claire Deo, Ahmed S. Abdelfattah, Hersh K. Bhargava, Adam J. Berro, Natalie Falco, View ORCID ProfileBenjamien Moeyaert, Mariam Chupanova, View ORCID ProfileLuke D. Lavis, View ORCID ProfileEric R. Schreiter
doi: https://doi.org/10.1101/2020.01.08.898783
Claire Deo
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USACell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
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Ahmed S. Abdelfattah
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
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Hersh K. Bhargava
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USABiophysics Graduate Program, University of California, San Francisco, San Francisco, CA, USA
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Adam J. Berro
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
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Natalie Falco
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
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Benjamien Moeyaert
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USADepartment of Chemistry, University of Leuven, Leuven, Belgium
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  • ORCID record for Benjamien Moeyaert
Mariam Chupanova
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
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Luke D. Lavis
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
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Eric R. Schreiter
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
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  • ORCID record for Eric R. Schreiter
  • For correspondence: schreitere@janelia.hhmi.org
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Abstract

Functional imaging using fluorescent indicators has revolutionized biology but additional sensor scaffolds are needed to access properties such as bright, far-red emission. We introduce a new platform for ‘chemigenetic’ fluorescent indicators, utilizing the self-labeling HaloTag protein conjugated to environmentally sensitive synthetic fluorophores. This approach affords bright, far-red calcium and voltage sensors with highly tunable photophysical and chemical properties, which can reliably detect single action potentials in neurons.

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Posted January 09, 2020.
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Bright and tunable far-red chemigenetic indicators
Claire Deo, Ahmed S. Abdelfattah, Hersh K. Bhargava, Adam J. Berro, Natalie Falco, Benjamien Moeyaert, Mariam Chupanova, Luke D. Lavis, Eric R. Schreiter
bioRxiv 2020.01.08.898783; doi: https://doi.org/10.1101/2020.01.08.898783
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Bright and tunable far-red chemigenetic indicators
Claire Deo, Ahmed S. Abdelfattah, Hersh K. Bhargava, Adam J. Berro, Natalie Falco, Benjamien Moeyaert, Mariam Chupanova, Luke D. Lavis, Eric R. Schreiter
bioRxiv 2020.01.08.898783; doi: https://doi.org/10.1101/2020.01.08.898783

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