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COP1 destabilizes DELLA proteins in Arabidopsis

Noel Blanco-Touriñán, Martina Legris, Eugenio G. Minguet, Cecilia Costigliolo-Rojas, María A. Nohales, Elisa Iniesto, Marta García-León, Manuel Pacín, Nicole Heucken, Tim Blomeier, Antonella Locascio, Martin Černý, David Esteve-Bruna, Mónica Díez-Díaz, Břetislav Brzobohatý, Henning Frerigmann, Matías D. Zurbriggen, Steve A. Kay, Vicente Rubio, Miguel A. Blázquez, Jorge J. Casal, View ORCID ProfileDavid Alabadí
doi: https://doi.org/10.1101/2020.01.09.897157
Noel Blanco-Touriñán
aInstituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politécnica de Valencia), Avda. de los Naranjos s/n, 46022 Valencia, Spain
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Martina Legris
bFundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires, CONICET, 1405 Buenos Aires, Argentina
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Eugenio G. Minguet
aInstituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politécnica de Valencia), Avda. de los Naranjos s/n, 46022 Valencia, Spain
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Cecilia Costigliolo-Rojas
bFundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires, CONICET, 1405 Buenos Aires, Argentina
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María A. Nohales
cDepartment of Neurology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90098, USA
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Elisa Iniesto
dDepartamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología (CNB-CSIC), Campus de Cantoblanco, Darwin 3, 28049 Madrid, Spain
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Marta García-León
dDepartamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología (CNB-CSIC), Campus de Cantoblanco, Darwin 3, 28049 Madrid, Spain
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Manuel Pacín
eIFEVA, Facultad de Agronomía, Universidad de Buenos Aires and CONICET, Av San Martín 4453, 1417 Buenos Aires, Argentina
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Nicole Heucken
fInstitute of Synthetic Biology and Cluster of Excellence in Plant Sciences (CEPLAS), University of Düsseldorf, 40225 Düsseldorf, Germany
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Tim Blomeier
fInstitute of Synthetic Biology and Cluster of Excellence in Plant Sciences (CEPLAS), University of Düsseldorf, 40225 Düsseldorf, Germany
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Antonella Locascio
aInstituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politécnica de Valencia), Avda. de los Naranjos s/n, 46022 Valencia, Spain
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Martin Černý
gDepartment of Molecular Biology and Radiobiology, CEITEC, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, CZ-61300 Brno, Czech Republic
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David Esteve-Bruna
aInstituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politécnica de Valencia), Avda. de los Naranjos s/n, 46022 Valencia, Spain
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Mónica Díez-Díaz
hUniversidad Católica de Valencia, Guillem de Castro 94, 46001-Valencia, Spain
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Břetislav Brzobohatý
gDepartment of Molecular Biology and Radiobiology, CEITEC, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, CZ-61300 Brno, Czech Republic
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Henning Frerigmann
iDepartment of Plant Microbe Interactions and Cluster of Excellence in Plant Sciences, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany
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Matías D. Zurbriggen
fInstitute of Synthetic Biology and Cluster of Excellence in Plant Sciences (CEPLAS), University of Düsseldorf, 40225 Düsseldorf, Germany
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Steve A. Kay
cDepartment of Neurology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90098, USA
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Vicente Rubio
dDepartamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología (CNB-CSIC), Campus de Cantoblanco, Darwin 3, 28049 Madrid, Spain
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Miguel A. Blázquez
aInstituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politécnica de Valencia), Avda. de los Naranjos s/n, 46022 Valencia, Spain
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Jorge J. Casal
bFundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires, CONICET, 1405 Buenos Aires, Argentina
eIFEVA, Facultad de Agronomía, Universidad de Buenos Aires and CONICET, Av San Martín 4453, 1417 Buenos Aires, Argentina
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  • For correspondence: dalabadi@ibmcp.upv.es casal@ifeva.edu.ar
David Alabadí
aInstituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politécnica de Valencia), Avda. de los Naranjos s/n, 46022 Valencia, Spain
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  • ORCID record for David Alabadí
  • For correspondence: dalabadi@ibmcp.upv.es casal@ifeva.edu.ar
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Abstract

DELLA transcriptional regulators are central components in the control of plant body form in response to the environment. This is considered to be mediated by changes in the metabolism of the hormones gibberellins (GAs), which promote the degradation of DELLAs. However, here we show that warm temperature or shade reduced the stability of a GA-insensitive DELLA allele in Arabidopsis. Furthermore, the degradation of DELLA induced by the warmth anticipated changes in GA levels and depended on the E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1). COP1 enhanced the degradation of normal and GA-insensitive DELLA alleles when co-expressed in N. benthamiana. DELLA proteins physically interacted with COP1 in yeast, mammalian and plant cells. This interaction was enhanced by the COP1 complex partner SUPRESSOR OF phyA-105 1 (SPA1). The level of ubiquitination of DELLA was enhanced by COP1 and COP1 ubiquitinated DELLA proteins in vitro. We propose that DELLAs are destabilized not only by the canonical GA-dependent pathway but also by COP1 and that this control is relevant for growth responses to shade and warm temperature.

Significance DELLA proteins are plant-specific transcriptional regulators that act as signaling hubs at the interface between the environment and the transcriptional networks that control growth. DELLAs are destabilized by the growth-promoting hormone gibberellin, whose levels are very sensitive to environmental changes. Here we describe an alternative pathway to destabilize these proteins. We show that DELLAs are substrate of COP1, an E3 ubiquitin ligase that increases its nuclear activity to promote growth in response to shade or warmth. Our results also show that the destabilization of DELLAs by COP1 precedes the action of gibberellins, suggesting the existence of a sequential mechanism to control the stability of these proteins.

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Posted January 09, 2020.
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COP1 destabilizes DELLA proteins in Arabidopsis
Noel Blanco-Touriñán, Martina Legris, Eugenio G. Minguet, Cecilia Costigliolo-Rojas, María A. Nohales, Elisa Iniesto, Marta García-León, Manuel Pacín, Nicole Heucken, Tim Blomeier, Antonella Locascio, Martin Černý, David Esteve-Bruna, Mónica Díez-Díaz, Břetislav Brzobohatý, Henning Frerigmann, Matías D. Zurbriggen, Steve A. Kay, Vicente Rubio, Miguel A. Blázquez, Jorge J. Casal, David Alabadí
bioRxiv 2020.01.09.897157; doi: https://doi.org/10.1101/2020.01.09.897157
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COP1 destabilizes DELLA proteins in Arabidopsis
Noel Blanco-Touriñán, Martina Legris, Eugenio G. Minguet, Cecilia Costigliolo-Rojas, María A. Nohales, Elisa Iniesto, Marta García-León, Manuel Pacín, Nicole Heucken, Tim Blomeier, Antonella Locascio, Martin Černý, David Esteve-Bruna, Mónica Díez-Díaz, Břetislav Brzobohatý, Henning Frerigmann, Matías D. Zurbriggen, Steve A. Kay, Vicente Rubio, Miguel A. Blázquez, Jorge J. Casal, David Alabadí
bioRxiv 2020.01.09.897157; doi: https://doi.org/10.1101/2020.01.09.897157

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