Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Cell-type specific impact of glucocorticoid receptor activation on the developing brain

View ORCID ProfileCristiana Cruceanu, Leander Dony, Anthi C. Krontira, David S. Fischer, Simone Roeh, Rossella Di Giaimo, Christina Kyrousi, Janine Arloth, Darina Czamara, Silvia Martinelli, Stefanie Wehner, Michael S. Breen, Maik Koedel, Susann Sauer, Monika Rex-Haffner, View ORCID ProfileSilvia Cappello, View ORCID ProfileFabian J. Theis, Elisabeth B. Binder
doi: https://doi.org/10.1101/2020.01.09.897868
Cristiana Cruceanu
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Cristiana Cruceanu
  • For correspondence: binder@psych.mpg.de cristiana_cruceanu@psych.mpg.de
Leander Dony
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
2International Max Planck Research School for Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
3Institute of Computational Biology, Helmholtz Zentrum München, 85764 Neuherberg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anthi C. Krontira
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
2International Max Planck Research School for Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David S. Fischer
3Institute of Computational Biology, Helmholtz Zentrum München, 85764 Neuherberg, Germany
4TUM School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Simone Roeh
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rossella Di Giaimo
5Max Planck Institute of Psychiatry, 80804 Munich, Germany
6Department of Biology, University of Naples Federico II, 80126, Naples, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christina Kyrousi
5Max Planck Institute of Psychiatry, 80804 Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Janine Arloth
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
3Institute of Computational Biology, Helmholtz Zentrum München, 85764 Neuherberg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Darina Czamara
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Silvia Martinelli
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stefanie Wehner
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael S. Breen
7Department of Psychiatry, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA
8Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA
9Seaver Autism Center for Research and Treatment, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA
10Pamela Sklar Division of Psychiatric Genomics, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maik Koedel
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susann Sauer
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Monika Rex-Haffner
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Silvia Cappello
5Max Planck Institute of Psychiatry, 80804 Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Silvia Cappello
Fabian J. Theis
3Institute of Computational Biology, Helmholtz Zentrum München, 85764 Neuherberg, Germany
11School of Life Sciences Weihenstephan, Technical University of Munich, Munich, Germany
12Department of Mathematics, Technical University of Munich, Munich, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Fabian J. Theis
Elisabeth B. Binder
1Department of Translational Psychiatry, Max Planck Institute of Psychiatry, 80804 Munich, Germany
13Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: binder@psych.mpg.de cristiana_cruceanu@psych.mpg.de
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

A fine-tuned balance of glucocorticoid receptor (GR) activation is essential for organ formation, with disturbances influencing health outcomes. Excess GR-activation in utero has been linked to brain-related negative outcomes, with unclear underlying mechanisms, especially regarding cell-type specific effects. To address this, we used an in vitro model of fetal human brain, induced pluripotent-stem-cell-derived cerebral organoids, and mapped GR-activation effects using single-cell transcriptomics across development. Interestingly, neurons showed targeted regulation of differentiation- and maturation-related transcripts, suggesting a delay of these processes upon GR-activation. Uniquely in neurons, differentially-expressed transcripts were significantly enriched for genes associated with behavior-related phenotypes and disorders. This suggests that aberrant GR-activation could impact proper neuronal maturation, leading to increased disease susceptibility, through neurodevelopmental processes at the interface of genetic susceptibility and environmental exposure.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted January 09, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Cell-type specific impact of glucocorticoid receptor activation on the developing brain
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Cell-type specific impact of glucocorticoid receptor activation on the developing brain
Cristiana Cruceanu, Leander Dony, Anthi C. Krontira, David S. Fischer, Simone Roeh, Rossella Di Giaimo, Christina Kyrousi, Janine Arloth, Darina Czamara, Silvia Martinelli, Stefanie Wehner, Michael S. Breen, Maik Koedel, Susann Sauer, Monika Rex-Haffner, Silvia Cappello, Fabian J. Theis, Elisabeth B. Binder
bioRxiv 2020.01.09.897868; doi: https://doi.org/10.1101/2020.01.09.897868
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Cell-type specific impact of glucocorticoid receptor activation on the developing brain
Cristiana Cruceanu, Leander Dony, Anthi C. Krontira, David S. Fischer, Simone Roeh, Rossella Di Giaimo, Christina Kyrousi, Janine Arloth, Darina Czamara, Silvia Martinelli, Stefanie Wehner, Michael S. Breen, Maik Koedel, Susann Sauer, Monika Rex-Haffner, Silvia Cappello, Fabian J. Theis, Elisabeth B. Binder
bioRxiv 2020.01.09.897868; doi: https://doi.org/10.1101/2020.01.09.897868

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4661)
  • Biochemistry (10315)
  • Bioengineering (7644)
  • Bioinformatics (26250)
  • Biophysics (13483)
  • Cancer Biology (10651)
  • Cell Biology (15366)
  • Clinical Trials (138)
  • Developmental Biology (8468)
  • Ecology (12778)
  • Epidemiology (2067)
  • Evolutionary Biology (16797)
  • Genetics (11373)
  • Genomics (15433)
  • Immunology (10583)
  • Microbiology (25089)
  • Molecular Biology (10172)
  • Neuroscience (54250)
  • Paleontology (398)
  • Pathology (1661)
  • Pharmacology and Toxicology (2884)
  • Physiology (4329)
  • Plant Biology (9216)
  • Scientific Communication and Education (1582)
  • Synthetic Biology (2545)
  • Systems Biology (6763)
  • Zoology (1459)