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Germ plasm localisation dynamics mark distinct phases of transcriptional and post-transcriptional regulation control in primordial germ cells

View ORCID ProfileFabio M. D’Orazio, Piotr Balwierz, Yixuan Guo, Benjamín Hernández-Rodríguez, Aleksandra Jasiulewicz, View ORCID ProfileJuan M. Vaquerizas, Bradley Cairns, Boris Lenhard, Ferenc Müller
doi: https://doi.org/10.1101/2020.01.12.903336
Fabio M. D’Orazio
1Institute of Cancer and Genomics Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
2MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK
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  • ORCID record for Fabio M. D’Orazio
Piotr Balwierz
1Institute of Cancer and Genomics Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
2MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK
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Yixuan Guo
3Department of Oncological Sciences and Huntsman Cancer Institute, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, UT, 84112, USA
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Benjamín Hernández-Rodríguez
4Max Planck Institute for Molecular Biomedicine, Roentgenstrasse 20, 48149 Muenster, Germany
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Aleksandra Jasiulewicz
1Institute of Cancer and Genomics Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
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Juan M. Vaquerizas
4Max Planck Institute for Molecular Biomedicine, Roentgenstrasse 20, 48149 Muenster, Germany
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  • ORCID record for Juan M. Vaquerizas
Bradley Cairns
3Department of Oncological Sciences and Huntsman Cancer Institute, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, UT, 84112, USA
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Boris Lenhard
2MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK
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  • For correspondence: f.mueller@bham.ac.uk b.lenhard@imperial.ac.uk
Ferenc Müller
1Institute of Cancer and Genomics Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
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  • For correspondence: f.mueller@bham.ac.uk b.lenhard@imperial.ac.uk
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SUMMARY

In many animal models, primordial germ cell (PGC) development depends on maternally-deposited germ plasm to avoid somatic cell fate. Here, we show that PGCs respond to regulatory information from the germ plasm in two distinct phases and mechanisms in zebrafish. We show that PGCs commence zygotic genome activation together with the rest of the embryo with no demonstrable differences in transcriptional and chromatin accessibility levels. Thus, cytoplasmic germ plasm determinants only affect post-transcriptional stabilisation of RNAs to diverge transcriptome from somatic cells, which, unexpectedly, also activate germ cell-specific genes. Perinuclear relocalisation of germ plasm is coupled to dramatic divergence in chromatin opening and transcriptome from somatic cells characterised by PGC-specific chromatin topology. Furthermore, we reveal Tdrd7, regulator of germ plasm localisation, as crucial determinant of germ fate acquisition.

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Posted January 14, 2020.
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Germ plasm localisation dynamics mark distinct phases of transcriptional and post-transcriptional regulation control in primordial germ cells
Fabio M. D’Orazio, Piotr Balwierz, Yixuan Guo, Benjamín Hernández-Rodríguez, Aleksandra Jasiulewicz, Juan M. Vaquerizas, Bradley Cairns, Boris Lenhard, Ferenc Müller
bioRxiv 2020.01.12.903336; doi: https://doi.org/10.1101/2020.01.12.903336
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Germ plasm localisation dynamics mark distinct phases of transcriptional and post-transcriptional regulation control in primordial germ cells
Fabio M. D’Orazio, Piotr Balwierz, Yixuan Guo, Benjamín Hernández-Rodríguez, Aleksandra Jasiulewicz, Juan M. Vaquerizas, Bradley Cairns, Boris Lenhard, Ferenc Müller
bioRxiv 2020.01.12.903336; doi: https://doi.org/10.1101/2020.01.12.903336

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