Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A calibrated optogenetic toolbox of stable zebrafish opsin lines

View ORCID ProfileP Antinucci, AS Dumitrescu, C Deleuze, HJ Morley, K Leung, T Hagley, F Kubo, View ORCID ProfileH Baier, View ORCID ProfileIH Bianco, View ORCID ProfileC Wyart
doi: https://doi.org/10.1101/2020.01.13.904185
P Antinucci
Department of Neuroscience, Physiology & Pharmacology, UCL, Gower Street, London, WC1E 6BT, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for P Antinucci
AS Dumitrescu
Institut du Cerveau et de la Moelle épinière (I.C.M.), Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, Hôpital Pitié-Salpêtrière, Paris, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
C Deleuze
Institut du Cerveau et de la Moelle épinière (I.C.M.), Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, Hôpital Pitié-Salpêtrière, Paris, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
HJ Morley
Department of Neuroscience, Physiology & Pharmacology, UCL, Gower Street, London, WC1E 6BT, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
K Leung
Department of Neuroscience, Physiology & Pharmacology, UCL, Gower Street, London, WC1E 6BT, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
T Hagley
Department of Neuroscience, Physiology & Pharmacology, UCL, Gower Street, London, WC1E 6BT, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
F Kubo
Department Genes – Circuits – Behavior, Max Planck Institute of Neurobiology, 82152 Martinsried, Germany.Center for Frontier Research, National Insitute of Genetics, 1111 Yata, Mishima, 411-8540, Japan.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
H Baier
Department Genes – Circuits – Behavior, Max Planck Institute of Neurobiology, 82152 Martinsried, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for H Baier
IH Bianco
Department of Neuroscience, Physiology & Pharmacology, UCL, Gower Street, London, WC1E 6BT, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for IH Bianco
  • For correspondence: claire.wyart@icm-institute.org i.bianco@ucl.ac.uk
C Wyart
Institut du Cerveau et de la Moelle épinière (I.C.M.), Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, Hôpital Pitié-Salpêtrière, Paris, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for C Wyart
  • For correspondence: claire.wyart@icm-institute.org i.bianco@ucl.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity with subcellular resolution and millisecond precision. Achieving reliable and interpretable in vivo optogenetic manipulations requires reproducible actuator expression and calibration of photocurrents in target neurons. Here, we developed nine transgenic zebrafish lines for stable opsin expression and calibrated their efficacy in vivo. We first used high-throughput behavioural assays to compare opsin ability to elicit or silence neural activity. Next, we performed in vivo whole-cell electrophysiological recordings to quantify the amplitude and kinetics of photocurrents and test opsin ability to precisely control spiking. We observed substantial variation in efficacy, associated with differences in both opsin expression level and photocurrent characteristics, and identified conditions for optimal use of the most efficient opsins. Overall, our calibrated optogenetic toolkit will facilitate the design of controlled optogenetic circuit manipulations.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted January 15, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A calibrated optogenetic toolbox of stable zebrafish opsin lines
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
A calibrated optogenetic toolbox of stable zebrafish opsin lines
P Antinucci, AS Dumitrescu, C Deleuze, HJ Morley, K Leung, T Hagley, F Kubo, H Baier, IH Bianco, C Wyart
bioRxiv 2020.01.13.904185; doi: https://doi.org/10.1101/2020.01.13.904185
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
A calibrated optogenetic toolbox of stable zebrafish opsin lines
P Antinucci, AS Dumitrescu, C Deleuze, HJ Morley, K Leung, T Hagley, F Kubo, H Baier, IH Bianco, C Wyart
bioRxiv 2020.01.13.904185; doi: https://doi.org/10.1101/2020.01.13.904185

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (1647)
  • Biochemistry (2739)
  • Bioengineering (1907)
  • Bioinformatics (10243)
  • Biophysics (4183)
  • Cancer Biology (3218)
  • Cell Biology (4539)
  • Clinical Trials (135)
  • Developmental Biology (2840)
  • Ecology (4461)
  • Epidemiology (2041)
  • Evolutionary Biology (7231)
  • Genetics (5477)
  • Genomics (6813)
  • Immunology (2390)
  • Microbiology (7485)
  • Molecular Biology (2993)
  • Neuroscience (18584)
  • Paleontology (136)
  • Pathology (472)
  • Pharmacology and Toxicology (780)
  • Physiology (1150)
  • Plant Biology (2706)
  • Scientific Communication and Education (680)
  • Synthetic Biology (888)
  • Systems Biology (2846)
  • Zoology (468)