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Selecting for Chlamydomonas reinhardtii fitness in a liquid algal growth system compatible with the International Space Station Veggie plant growth chamber

View ORCID ProfileJunya Zhang, View ORCID ProfileBárbara S.F. Müller, View ORCID ProfileKevin N. Tyre, View ORCID ProfileFang Bai, Ying Hu, Marcio F.R. Resende Jr., View ORCID ProfileBala Rathinasabapathi, View ORCID ProfileA. Mark Settles
doi: https://doi.org/10.1101/2020.01.13.904342
Junya Zhang
1Horticultural Sciences Department, University of Florida, Gainesville, FL, USA
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Bárbara S.F. Müller
1Horticultural Sciences Department, University of Florida, Gainesville, FL, USA
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Kevin N. Tyre
2Center for the Advancement of Science in Space, Melbourne, FL, USA
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Fang Bai
1Horticultural Sciences Department, University of Florida, Gainesville, FL, USA
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Ying Hu
1Horticultural Sciences Department, University of Florida, Gainesville, FL, USA
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Marcio F.R. Resende Jr.
1Horticultural Sciences Department, University of Florida, Gainesville, FL, USA
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Bala Rathinasabapathi
1Horticultural Sciences Department, University of Florida, Gainesville, FL, USA
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A. Mark Settles
1Horticultural Sciences Department, University of Florida, Gainesville, FL, USA
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  • For correspondence: settles@ufl.edu
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Abstract

A biological life support system for spaceflight would capture carbon dioxide waste produced by living and working in space to generate useful organic compounds. Photosynthesis is the primary mechanism to fix carbon into organic molecules. Microalgae are highly efficient at converting light, water, and carbon dioxide into biomass, particularly under limiting, artificial light conditions that are a necessity in space photosynthetic production. Although there is great promise in developing algae for chemical or food production in space, most spaceflight algae growth studies have been conducted on solid agar-media to avoid handling liquids in microgravity. Here we report that breathable plastic tissue culture bags can support robust growth of Chlamydomonas reinhardtii in the Veggie plant growth chamber, which is used on the International Space Station to grow terrestrial plants. Live cultures can be stored for at least one month in the bags at room temperature. The gene set required for growth in these photobioreactors was tested through a short-wave ultraviolet light (UVC) mutagenesis and selection experiment with wild-type (CC-5082) and cw15 mutant (CC-1883) strains. Genome sequencing identified UVC-induced mutations, which were enriched for transversions and nonsynonymous mutations relative to natural variants among laboratory strains. Genes with mutations indicating positive selection were enriched for information processing genes related to DNA repair, RNA processing, translation, cytoskeletal motors, kinases, and ABC transporters. These data suggest modification of signal transduction and metabolite transport may be needed to improve growth rates in this spaceflight production system.

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  • https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-265/

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Posted January 14, 2020.
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Selecting for Chlamydomonas reinhardtii fitness in a liquid algal growth system compatible with the International Space Station Veggie plant growth chamber
Junya Zhang, Bárbara S.F. Müller, Kevin N. Tyre, Fang Bai, Ying Hu, Marcio F.R. Resende Jr., Bala Rathinasabapathi, A. Mark Settles
bioRxiv 2020.01.13.904342; doi: https://doi.org/10.1101/2020.01.13.904342
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Selecting for Chlamydomonas reinhardtii fitness in a liquid algal growth system compatible with the International Space Station Veggie plant growth chamber
Junya Zhang, Bárbara S.F. Müller, Kevin N. Tyre, Fang Bai, Ying Hu, Marcio F.R. Resende Jr., Bala Rathinasabapathi, A. Mark Settles
bioRxiv 2020.01.13.904342; doi: https://doi.org/10.1101/2020.01.13.904342

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