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Hedgehog signaling is required for endomesodermal patterning and germ cell development in Nematostella vectensis

Cheng-Yi Chen, Sean A. McKinney, Lacey R. Ellington, View ORCID ProfileMatthew C. Gibson
doi: https://doi.org/10.1101/2020.01.15.907238
Cheng-Yi Chen
1Stowers Institute for Medical Research, Kansas City, MO 64110, USA
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Sean A. McKinney
1Stowers Institute for Medical Research, Kansas City, MO 64110, USA
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Lacey R. Ellington
1Stowers Institute for Medical Research, Kansas City, MO 64110, USA
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Matthew C. Gibson
1Stowers Institute for Medical Research, Kansas City, MO 64110, USA
2Department of Anatomy and Cell Biology, The University of Kansas School of Medicine, Kansas City, KS 66160, USA
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  • ORCID record for Matthew C. Gibson
  • For correspondence: mg2@stowers.org
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Abstract

Two distinct mechanisms for primordial germ cell (PGC) specification are observed within Bilatera: early determination by maternal factors or late induction by zygotic cues. Here we investigate the molecular basis for PGC specification in Nematostella, a representative pre-bilaterian animal where PGCs arise as paired endomesodermal cell clusters during early development. We first present evidence that the putative PGCs delaminate from the endomesoderm upon feeding, migrate into the gonad primordia, and mature into germ cells. We then show that the PGC clusters arise at the interface between hedgehog1 and patched domains in the developing mesenteries and use gene knockdown, knockout and inhibitor experiments to demonstrate that Hh signaling is required for both PGC specification and general endomesodermal patterning. These results provide evidence that the Nematostella germline is specified by inductive signals rather than maternal factors, and support the existence of zygotically-induced PGCs in the eumetazoan common ancestor.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted January 15, 2020.
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Hedgehog signaling is required for endomesodermal patterning and germ cell development in Nematostella vectensis
Cheng-Yi Chen, Sean A. McKinney, Lacey R. Ellington, Matthew C. Gibson
bioRxiv 2020.01.15.907238; doi: https://doi.org/10.1101/2020.01.15.907238
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Hedgehog signaling is required for endomesodermal patterning and germ cell development in Nematostella vectensis
Cheng-Yi Chen, Sean A. McKinney, Lacey R. Ellington, Matthew C. Gibson
bioRxiv 2020.01.15.907238; doi: https://doi.org/10.1101/2020.01.15.907238

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