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Standardized and reproducible measurement of decision-making in mice

The International Brain Laboratory, Valeria Aguillon-Rodriguez, View ORCID ProfileDora E. Angelaki, View ORCID ProfileHannah M. Bayer, View ORCID ProfileNiccolò Bonacchi, View ORCID ProfileMatteo Carandini, View ORCID ProfileFanny Cazettes, View ORCID ProfileGaelle A. Chapuis, View ORCID ProfileAnne K. Churchland, View ORCID ProfileYang Dan, Eric E. J. Dewitt, Mayo Faulkner, Hamish Forrest, View ORCID ProfileLaura M. Haetzel, View ORCID ProfileMichael Hausser, View ORCID ProfileSonja B. Hofer, View ORCID ProfileFei Hu, View ORCID ProfileAnup Khanal, View ORCID ProfileChristopher S. Krasniak, Inês Laranjeira, View ORCID ProfileZachary F. Mainen, View ORCID ProfileGuido T. Meijer, View ORCID ProfileNathaniel J. Miska, View ORCID ProfileThomas D. Mrsic-Flogel, Masayoshi Murakami, View ORCID ProfileJean-Paul Noel, Alejandro Pan-Vazquez, Cyrille Rossant, Joshua I. Sanders, View ORCID ProfileKarolina Z. Socha, Rebecca Terry, View ORCID ProfileAnne E. Urai, View ORCID ProfileHernando M. Vergara, View ORCID ProfileMiles J. Wells, Christian J. Wilson, View ORCID ProfileIlana B. Witten, View ORCID ProfileLauren E. Wool, View ORCID ProfileAnthony Zador
doi: https://doi.org/10.1101/2020.01.17.909838
1Cold Spring Harbor Laboratory, NY, USA
  • For correspondence: info+behavior@internationalbrainlab.org
Valeria Aguillon-Rodriguez
1Cold Spring Harbor Laboratory, NY, USA
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Dora E. Angelaki
2Center for Neural Science, New York University, NY, USA
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Hannah M. Bayer
3Zuckerman Institute, Columbia University, NY USA
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Niccolò Bonacchi
4Champalimaud Centre for the Unknown, Lisbon, Portugal
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Matteo Carandini
5UCL Institute of Ophthalmology, University College London, London, UK
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Fanny Cazettes
4Champalimaud Centre for the Unknown, Lisbon, Portugal
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Gaelle A. Chapuis
6Wolfson Institute for Biomedical Research, University College London, London, UK
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Anne K. Churchland
1Cold Spring Harbor Laboratory, NY, USA
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Yang Dan
7Dept. of Molecular and Cell Biology, University of California, Berkeley, CA, USA
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Eric E. J. Dewitt
4Champalimaud Centre for the Unknown, Lisbon, Portugal
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Mayo Faulkner
6Wolfson Institute for Biomedical Research, University College London, London, UK
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Hamish Forrest
5UCL Institute of Ophthalmology, University College London, London, UK
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Laura M. Haetzel
8Princeton Neuroscience Institute, Princeton University, NJ, USA
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Michael Hausser
6Wolfson Institute for Biomedical Research, University College London, London, UK
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Sonja B. Hofer
9Sainsbury-Wellcome Centre for Neural Circuits and Behaviour, University College London, London, UK
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Fei Hu
7Dept. of Molecular and Cell Biology, University of California, Berkeley, CA, USA
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Anup Khanal
1Cold Spring Harbor Laboratory, NY, USA
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Christopher S. Krasniak
1Cold Spring Harbor Laboratory, NY, USA
10Watson School of Biological Sciences, NY, USA
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  • ORCID record for Christopher S. Krasniak
Inês Laranjeira
4Champalimaud Centre for the Unknown, Lisbon, Portugal
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Zachary F. Mainen
4Champalimaud Centre for the Unknown, Lisbon, Portugal
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Guido T. Meijer
4Champalimaud Centre for the Unknown, Lisbon, Portugal
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Nathaniel J. Miska
9Sainsbury-Wellcome Centre for Neural Circuits and Behaviour, University College London, London, UK
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Thomas D. Mrsic-Flogel
9Sainsbury-Wellcome Centre for Neural Circuits and Behaviour, University College London, London, UK
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Masayoshi Murakami
4Champalimaud Centre for the Unknown, Lisbon, Portugal
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Jean-Paul Noel
2Center for Neural Science, New York University, NY, USA
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Alejandro Pan-Vazquez
8Princeton Neuroscience Institute, Princeton University, NJ, USA
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Cyrille Rossant
12UCL Queen Square Institute of Neurology, University College London, London, UK
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Joshua I. Sanders
11Sanworks LLC, NY, USA
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Karolina Z. Socha
5UCL Institute of Ophthalmology, University College London, London, UK
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Rebecca Terry
12UCL Queen Square Institute of Neurology, University College London, London, UK
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Anne E. Urai
1Cold Spring Harbor Laboratory, NY, USA
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Hernando M. Vergara
9Sainsbury-Wellcome Centre for Neural Circuits and Behaviour, University College London, London, UK
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Miles J. Wells
12UCL Queen Square Institute of Neurology, University College London, London, UK
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Christian J. Wilson
2Center for Neural Science, New York University, NY, USA
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Ilana B. Witten
8Princeton Neuroscience Institute, Princeton University, NJ, USA
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Lauren E. Wool
12UCL Queen Square Institute of Neurology, University College London, London, UK
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Anthony Zador
1Cold Spring Harbor Laboratory, NY, USA
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Abstract

Progress in science requires standardized assays whose results can be readily shared, compared, and reproduced across laboratories. Reproducibility, however, has been a concern in neuroscience, particularly for measurements of mouse behavior. Here we show that a standardized task to probe decision-making in mice produces reproducible results across multiple laboratories. We designed a task for head-fixed mice that combines established assays of perceptual and value-based decision making, and we standardized training protocol and experimental hardware, software, and procedures. We trained 140 mice across seven laboratories in three countries, and we collected 5 million mouse choices into a publicly available database. Learning speed was variable across mice and laboratories, but once training was complete there were no significant differences in behavior across laboratories. Mice in different laboratories adopted similar reliance on visual stimuli, on past successes and failures, and on estimates of stimulus prior probability to guide their choices. These results reveal that a complex mouse behavior can be successfully reproduced across multiple laboratories. They establish a standard for reproducible rodent behavior, and provide an unprecedented dataset and open-access tools to study decision-making in mice. More generally, they indicate a path towards achieving reproducibility in neuroscience through collaborative open-science approaches.

Competing Interest Statement

J.I.S. is the owner of Sanworks LLC which provides hardware and consulting for the experimental set-up described in this work.

Footnotes

  • https://data.internationalbrainlab.org

  • https://github.com/int-brain-lab

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Standardized and reproducible measurement of decision-making in mice
The International Brain Laboratory, Valeria Aguillon-Rodriguez, Dora E. Angelaki, Hannah M. Bayer, Niccolò Bonacchi, Matteo Carandini, Fanny Cazettes, Gaelle A. Chapuis, Anne K. Churchland, Yang Dan, Eric E. J. Dewitt, Mayo Faulkner, Hamish Forrest, Laura M. Haetzel, Michael Hausser, Sonja B. Hofer, Fei Hu, Anup Khanal, Christopher S. Krasniak, Inês Laranjeira, Zachary F. Mainen, Guido T. Meijer, Nathaniel J. Miska, Thomas D. Mrsic-Flogel, Masayoshi Murakami, Jean-Paul Noel, Alejandro Pan-Vazquez, Cyrille Rossant, Joshua I. Sanders, Karolina Z. Socha, Rebecca Terry, Anne E. Urai, Hernando M. Vergara, Miles J. Wells, Christian J. Wilson, Ilana B. Witten, Lauren E. Wool, Anthony Zador
bioRxiv 2020.01.17.909838; doi: https://doi.org/10.1101/2020.01.17.909838
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Standardized and reproducible measurement of decision-making in mice
The International Brain Laboratory, Valeria Aguillon-Rodriguez, Dora E. Angelaki, Hannah M. Bayer, Niccolò Bonacchi, Matteo Carandini, Fanny Cazettes, Gaelle A. Chapuis, Anne K. Churchland, Yang Dan, Eric E. J. Dewitt, Mayo Faulkner, Hamish Forrest, Laura M. Haetzel, Michael Hausser, Sonja B. Hofer, Fei Hu, Anup Khanal, Christopher S. Krasniak, Inês Laranjeira, Zachary F. Mainen, Guido T. Meijer, Nathaniel J. Miska, Thomas D. Mrsic-Flogel, Masayoshi Murakami, Jean-Paul Noel, Alejandro Pan-Vazquez, Cyrille Rossant, Joshua I. Sanders, Karolina Z. Socha, Rebecca Terry, Anne E. Urai, Hernando M. Vergara, Miles J. Wells, Christian J. Wilson, Ilana B. Witten, Lauren E. Wool, Anthony Zador
bioRxiv 2020.01.17.909838; doi: https://doi.org/10.1101/2020.01.17.909838

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